Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1996-11-20
|
| pubmed:abstractText |
HLA class I antigens of the human major histocompatibility complex play an important role in immune response. These molecules present foreign antigenic peptides to cytotoxic T lymphocytes and thereby play a role in the immune surveillance of cells infected with virus or other intracellular pathogens or altered by malignant transformation. A marked deficiency or lack of expression of these antigens has been reported in a variety of human neoplasms. In the present study, we examined the expression of class I alpha chain, beta 2-microglobulin, TAP (TAP1 and TAP2) and LMP (LMP2 and LMP7) genes in a number of human tumor cell lines including small-cell lung carcinoma, hepatocellular carcinoma, colon adenocarcinoma and basophilic leukaemia. These cell lines were deficient in expression of both class I alpha chain and beta 2-microglobulin gene products. In addition, these cell lines lacked the products of MHC-encoded proteasome subunit LMP2 as well as the putative peptide transporter TAP1 genes. In contrast, TAP2 and LMP7 genes were expressed in these cell lines. Treatment of cells with gamma-IFN markedly enhanced the expression of class I alpha chain, beta 2-microglobulin, TAP1 and LMP2 genes with a concomitant increase in cell-surface expression of class I molecules. The upregulation of TAP1 and LMP2 expression is associated with increased class I expression, suggesting that endogenous antigens, e.g. tumor antigens, could be presented by class I molecules following treatment of tumor cells with gamma-IFN.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ATP-Binding Cassette Transporters,
http://linkedlifedata.com/resource/pubmed/chemical/Cysteine Endopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens Class I,
http://linkedlifedata.com/resource/pubmed/chemical/LMP-2 protein,
http://linkedlifedata.com/resource/pubmed/chemical/LMP7 protein,
http://linkedlifedata.com/resource/pubmed/chemical/Multienzyme Complexes,
http://linkedlifedata.com/resource/pubmed/chemical/Proteasome Endopeptidase Complex,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/TAP1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/TAP2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/beta 2-Microglobulin
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0165-2478
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
50
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
149-54
|
| pubmed:dateRevised |
2010-11-18
|
| pubmed:meshHeading |
pubmed-meshheading:8803612-ATP-Binding Cassette Transporters,
pubmed-meshheading:8803612-Cysteine Endopeptidases,
pubmed-meshheading:8803612-Down-Regulation,
pubmed-meshheading:8803612-Flow Cytometry,
pubmed-meshheading:8803612-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:8803612-Histocompatibility Antigens Class I,
pubmed-meshheading:8803612-Humans,
pubmed-meshheading:8803612-Multienzyme Complexes,
pubmed-meshheading:8803612-Neoplasms,
pubmed-meshheading:8803612-Proteasome Endopeptidase Complex,
pubmed-meshheading:8803612-Proteins,
pubmed-meshheading:8803612-Tumor Cells, Cultured,
pubmed-meshheading:8803612-beta 2-Microglobulin
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Markedly decreased expression of TAP1 and LMP2 genes in HLA class I-deficient human tumor cell lines.
|
| pubmed:affiliation |
Department of Pathology, McMaster University, Hamilton, Ontario, Canada.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|