Linked Life Data

8798905

Source:http://linkedlifedata.com/resource/pubmed/id/8798905

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1997-1-23
pubmed:abstractText
Cell wall storage polysaccharides from Brazilian legume seeds of Dimorphandra mollis, Schizolobium parahybum (galactomannans), Copaifera langsdorffii, Hymenaea courbaril (xyloglucans) and the galactan from cotyledons of the Mediterranean species Lupinus angustifolius were extracted and their apparent molecular masses were determined by high-performance size exclusion chromatography analysis. They were, to a large degree, polydisperse, showing molecular masses that varied from 100,000 to 2,000,000. Polyethylene glycol (PEG, 1500, 4000, 6000 and 8000), sodium citrate and dextran (73,000, 60,000-90,000, 505,000 and 2,000,000) were used for investigating phase formation with the seed polysaccharides. Galactomannans and xyloglucans demonstrated phase formation with sodium citrate concentrations lower than 30%, as well as dextrans and polyethylene glycol, and formed gels in the presence of high concentrations of sodium citrate (above 30%). Galactan did not promote phase formation with any of the reagents used. On the basis of the results obtained, the possibility of using legume seed polysaccharides for the partitioning and purification of polysaccharide enzymes in aqueous two-phase systems is suggested.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1572-6495
pubmed:author
pubmed:issnType
Print
pubmed:day
17
pubmed:volume
680
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
255-61
pubmed:dateRevised
2007-10-16
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Characterization of storage cell wall polysaccharides from Brazilian legume seeds and the formation of aqueous two-phase systems.
pubmed:affiliation
CPQBA-UNICAMP, Campinas, Brazil.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't