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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1996-10-21
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pubmed:abstractText |
Human adenocarcinoma cells of the line WiDr and Chinese hamster lung fibroblasts of the line V79 were treated with 5-aminolaevulinic acid (5-ALA) and exposed to light. The effects of the iron chelators ethylenediaminetetraacetic acid (EDTA) and desferrioxamine (DEF) were assessed. Both cell lines were treated with various concentrations of 5-ALA in the presence or absence of the iron chelators for 4 h in serum-free medium. The accumulation of protoporphyrin IX (PpIX) reached a maximum level at 1 mM 5-ALA in WiDr cells [280 ng PpIX (mg protein x 4 h-1] and at 0.1 mM 5-ALA in V79 cells [55 ng PpIX (mg protein x 4 h)-1]. PpIX was the only fluorescing porphyrin in these cells after 5-ALA treatment alone or in combination with the chelators. The iron chelators did not influence the intracellular localisation pattern of PpIX in 5-ALA-treated cells. While both chelators enhanced the accumulation of PpIX in 5-ALA-treated cells, DEF was found to be superior at equal concentrations. A linear relationship between the applied concentration of DEF and the DEF-induced increase in PpIX accumulation was observed in double-reciprocal plots. The intercepts of the regression lines with the ordinate indicate that the ferrochelatase is saturated with PpIX when the 5-ALA concentration exceeds 0.3 mM and 0.05 mM in WiDr and V79 cells respectively. The DEF-induced enhancement of PpIX accumulation in 5-ALA-treated cells was cell line and 5-ALA concentration dependent. At a 5-ALA concentration inducing a maximum level of PpIX accumulation, inhibition of ferrochelatase activity enhanced the PpIX accumulation 3- and 1.4-fold in V79 and WiDr cells respectively. The relative gain in PpIX accumulation increased with decreasing concentration of 5-ALA. In cells treated with the lowest concentrations of 5-ALA used in this study, DEF enhanced PpIX accumulation 44- and 3.5-fold in V79 and WiDr cells respectively. The iron chelator-induced increase in cellular PpIX accumulation was followed by a similar increase in sensitivity to photoinactivation. The ferrochelatase inhibitor dihydropyridine 3,5-diethoxycarbonyl-1,4-dihydrocollidine reduced the accumulation of PpIX in both cell lines.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8795569-1238396,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8795569-1249866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8795569-1386052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8795569-1403373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8795569-1409894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8795569-14219455,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8795569-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8795569-1497480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8795569-1511416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8795569-1513328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8795569-1558795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8795569-1561241,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8795569-1603846,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8795569-1931609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8795569-2011624,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8795569-2121931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8795569-2340297,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8795569-265562,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8795569-3203329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8795569-328083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8795569-3480752,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8795569-6073903,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8795569-6497856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8795569-6894597,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8795569-7173190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8795569-7638255,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8795569-7770514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8795569-7840508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8795569-8018536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8795569-8262664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8795569-8306330,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8795569-8308010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8795569-8492244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8795569-90012
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0007-0920
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
74
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
688-97
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8795569-Adenocarcinoma,
pubmed-meshheading:8795569-Aminolevulinic Acid,
pubmed-meshheading:8795569-Animals,
pubmed-meshheading:8795569-Colonic Neoplasms,
pubmed-meshheading:8795569-Cricetinae,
pubmed-meshheading:8795569-Cricetulus,
pubmed-meshheading:8795569-Dicarbethoxydihydrocollidine,
pubmed-meshheading:8795569-Edetic Acid,
pubmed-meshheading:8795569-Fibroblasts,
pubmed-meshheading:8795569-Humans,
pubmed-meshheading:8795569-Iron Chelating Agents,
pubmed-meshheading:8795569-Microscopy, Fluorescence,
pubmed-meshheading:8795569-Photosensitizing Agents,
pubmed-meshheading:8795569-Protoporphyrins,
pubmed-meshheading:8795569-Thiophosphoric Acid Esters,
pubmed-meshheading:8795569-Tumor Cells, Cultured
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pubmed:year |
1996
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pubmed:articleTitle |
The influence of iron chelators on the accumulation of protoporphyrin IX in 5-aminolaevulinic acid-treated cells.
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pubmed:affiliation |
Institute for Cancer Research, Department of Biophysics, Montebello, Norway.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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