Linked Life Data

8795215

Source:http://linkedlifedata.com/resource/pubmed/id/8795215

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1996-10-17
pubmed:abstractText
Aflatoxins are carcinogenic metabolites produced by several members of the Aspergillus flavus group in grains and floods. Three genes, ver-1, omt-1, and apa-2, coding for key enzymes and a regulatory factor in aflatoxin biosynthesis, respectively, have been identified, and their DNA sequences have been published. In the present study, three primer pairs, each complementing the coding portion of one of the genes, were generated. DNA extracted from mycelia of five Aspergillus species, four Penicillium species, and two Fusarium species was used as PCR template for each of the primer pairs. DNA extracted from peanut, corn, and three insect species commonly found in stored grains was also tested. Positive results (DNA amplification) were achieved only with DNA of the aflatoxigenic molds Aspergillus parasiticus and A. flavus in all three primer pairs. The detection limit of the PCR was determined by using the primer pairs complementing the omt-1 and ver-1 genes. Sterile corn flour was inoculated separately with six different molds, each at several spore concentrations. Positive results were obtained only after a 24-h incubation in enriched media, with extracts of corn inoculated with A. parasiticus or A. flavus, even at the lowest spore concentration applied (10(2) spores per g). No DNA spores per g). It is concluded that genes involved in the aflatoxin biosynthetic pathway may form the basis for an accurate, sensitive, and specific detection system, using PCR, for aflatoxigenic strains in grains and foods.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8795215-1339261, http://linkedlifedata.com/resource/pubmed/commentcorrection/8795215-1379788, http://linkedlifedata.com/resource/pubmed/commentcorrection/8795215-1563048, http://linkedlifedata.com/resource/pubmed/commentcorrection/8795215-1910523, http://linkedlifedata.com/resource/pubmed/commentcorrection/8795215-2515438, http://linkedlifedata.com/resource/pubmed/commentcorrection/8795215-7758228, http://linkedlifedata.com/resource/pubmed/commentcorrection/8795215-7793909, http://linkedlifedata.com/resource/pubmed/commentcorrection/8795215-7793957, http://linkedlifedata.com/resource/pubmed/commentcorrection/8795215-8215366, http://linkedlifedata.com/resource/pubmed/commentcorrection/8795215-8250554, http://linkedlifedata.com/resource/pubmed/commentcorrection/8795215-8285664, http://linkedlifedata.com/resource/pubmed/commentcorrection/8795215-8382024, http://linkedlifedata.com/resource/pubmed/commentcorrection/8795215-8439147
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
62
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3270-3
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Detection of aflatoxigenic molds in grains by PCR.
pubmed:affiliation
Institute of Biochemistry, Food Science, and Nutrition, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot, Israel.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't