Linked Life Data

8795002

Source:http://linkedlifedata.com/resource/pubmed/id/8795002

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1996-12-4
pubmed:abstractText
A simple, convenient method employing a gE-specific neutralizing monoclonal antibody (mAb; FTpn3) for isolation of the gE gene-deleted recombinant pseudorabies virus (PRV) is described. FTpn3 secreting hybridoma was obtained by fusing PRV-immunized BALB/c splenocytes with myeloma cells. To construct gE gene deleted PRV, a 5.7 kbp DNA fragment with deletion of the gE gene was engineered and cloned. The plasmid was then used for cotransfecting Vero cells with wild-type PRV genome. The resulting viruses were subjected to FTpn3 neutralization. The FTpn3 resistant virus was isolated and plaque purified further. By DNA fingerprinting and Western blotting analysis, the virus resistant to FTpn3 neutralization was proved to be the gE-deleted recombinant virus.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0166-0934
pubmed:author
pubmed:issnType
Print
pubmed:volume
60
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
19-28
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Isolation of gE gene deleted pseudorabies virus by using a gE specific monoclonal antibody.
pubmed:affiliation
Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't