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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1996-11-22
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pubmed:abstractText |
Studies of human T-cell leukemia virus type 1 (HTLV-1) have been hampered by the difficulty of achieving high cell-free and cell-associated infectious titers. Current retroviral pseudotyping systems using the HTLV-1 envelope generate titers of less than 200 infectious particles per ml. We describe here an improved system for pseudotyping using a defective human immunodeficiency virus (HIV) type 1 genome in combination with HTLV-1 env in 293T producer cells. Introduction of additional copies of rev and treatment of cells with sodium butyrate resulted in a cell-associated titer of 10(5)/ml and cell-free titers of greater than 10(4)/ml . By using this system, we found that the host range of HTLV-1 is even greater than previously suspected. Earlier studies which assigned a chromosomal location for the HTLV-1 receptor may therefore reflect cell-to-cell variation in receptor number rather than the absolute presence or absence of a receptor. The generation of higher-titer HIV(HTLV-1) may facilitate identification of the cellular receptor and investigations of the pathophysiology of HTLV-1 infection.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-1056809,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-1401919,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-1691887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-1845835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-1845882,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-1883541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-2078500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-2406030,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-2541919,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-2677251,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-276864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-2784194,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-2784836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-2825693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-2863442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-2881513,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-2896300,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-2982877,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-2983944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-3201246,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-3299749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-3499186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-4308055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-6095309,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-6174854,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-6261256,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-6316266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-6316502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-6329966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-649576,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-6604033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-6979048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-6980846,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-7690960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-7769729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-7884847,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-7899083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-7941334,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-8057442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-8278411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-8383211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-8402880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-8416382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794391-8615036
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
70
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7322-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1996
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pubmed:articleTitle |
Broad host range of human T-cell leukemia virus type 1 demonstrated with an improved pseudotyping system.
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pubmed:affiliation |
Howard Hughes Medical Institute, Stanford University, California 94305, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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