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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1996-11-22
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pubmed:abstractText |
The Gag-Pol polyprotein of human immunodeficiency virus type 1 is not required for efficient viral particle assembly or release. However, in this report we demonstrate that the synthesis of a truncated Gag-Pol precursor due to a premature termination codon in pol can reduce the ability of a full-length provirus to direct the formation of viral particles. Marked effects on particle production were seen when premature termination codons were introduced into the integrase (IN)-coding region. By contrast, a mutant which lacked both IN and reverse transcriptase (RT) formed particles with normal efficiency. Particle production by IN mutants was restored to wild-type levels when a second premature termination codon was introduced at the 5' end of the RT-coding sequence. Particle formation was similarly restored by a second site mutation in the viral protease (PR) gene which prevented proteolytic processing of the Gag polyprotein. Finally particle formation was restored in the presence of A77003, a specific inhibitor of human immunodeficiency virus type 1 PR. These results suggest that the effects of a lack of IN sequences on particle formation require the synthesis of a Gag-Pol precursor which contains RT sequences and are due to inappropriate PR activity.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794322-1652977,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794322-1706590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794322-1803993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794322-1870215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794322-1883539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794322-2014240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794322-2014242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794322-2078037,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794322-2157898,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794322-2203609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794322-2394131,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794322-2405382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794322-2447506,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794322-2676191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794322-2788277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794322-3060262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794322-3262776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794322-3290901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794322-3323813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794322-3657593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794322-7474093,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794322-7535863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794322-7544046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794322-7585960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794322-7666503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794322-7707554,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794322-7969495,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794322-7983732,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794322-8030280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794322-8035478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794322-8035491,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794322-8083996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794322-8107224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794322-8346259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794322-8551608,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8794322-8709190
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
70
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6820-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8794322-Animals,
pubmed-meshheading:8794322-Base Sequence,
pubmed-meshheading:8794322-Cricetinae,
pubmed-meshheading:8794322-Endopeptidases,
pubmed-meshheading:8794322-HIV Protease Inhibitors,
pubmed-meshheading:8794322-HIV-1,
pubmed-meshheading:8794322-Humans,
pubmed-meshheading:8794322-Integrases,
pubmed-meshheading:8794322-Methylurea Compounds,
pubmed-meshheading:8794322-Molecular Sequence Data,
pubmed-meshheading:8794322-Mutation,
pubmed-meshheading:8794322-Pyridines,
pubmed-meshheading:8794322-Virus Assembly,
pubmed-meshheading:8794322-Virus Integration
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pubmed:year |
1996
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pubmed:articleTitle |
Lack of integrase can markedly affect human immunodeficiency virus type 1 particle production in the presence of an active viral protease.
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pubmed:affiliation |
Division of Human Retrovirology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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