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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
1996-11-7
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pubmed:abstractText |
Two-dimensional HPLC mapping of O-linked sugar chains was studied. Twenty-seven pyridylamino (PA-) derivatives of O-linked sugar chains liberated from glycoproteins by hydrazinolysis-N-acetylation were separated by reversed-phase and size-fractionation HPLC. Combining the elution positions obtained by these two HPLC techniques allows the construction of a two-dimensional sugar map which can be used to analyze the structure of PA-O-linked sugar chains. As in the case of PA-N-linked sugar chains, the additivity rule which correlates chemical structures with elution positions was also found in the case of PA-O-linked sugar chains. This separation system was effectively applied to the elucidation of minute amounts of PA-O-linked oligosaccharides.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
|
pubmed:issn |
0003-2697
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
233
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
205-11
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8789719-Aminopyridines,
pubmed-meshheading:8789719-Animals,
pubmed-meshheading:8789719-Carbohydrate Sequence,
pubmed-meshheading:8789719-Carbohydrates,
pubmed-meshheading:8789719-Cattle,
pubmed-meshheading:8789719-Chromatography, High Pressure Liquid,
pubmed-meshheading:8789719-Humans,
pubmed-meshheading:8789719-Molecular Sequence Data,
pubmed-meshheading:8789719-Swine
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pubmed:year |
1996
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pubmed:articleTitle |
Analysis of pyridylaminated O-linked sugar chains by two-dimensional sugar mapping.
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pubmed:affiliation |
Department of Chemistry, Osaka University College of Science, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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