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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1996-10-22
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pubmed:abstractText |
In the pediatric cancer alveolar rhabdomyosarcoma, characteristic t(2;13)(q35;q14) or variant t(1;13)(p36;q14) chromosomal translocations generate PAX3-FKHR or PAX7-FKHR fusion genes. Using fluorescence in situ hybridization, reverse transcriptase-polymerase chain reaction and quantitative Southern blot analyses, we demonstrate that these fusion genes are amplified in 20% of fusion-positive tumors. In particular, we found in vivo amplification of these fusions in one of 22 PAX3-FKHR-positive cases and five of seven PAX7-FKHR-positive cases. These findings indicate that translocation and amplification can occur sequentially in a cancer to alter both the structure and copy number of a gene and thereby activate oncogenic activity by complementary mechanisms.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/FOXO1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Forkhead Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Muscle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/PAX3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/PAX7 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/PAX7 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Paired Box Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Pax3 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0964-6906
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
15-21
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pubmed:dateRevised |
2008-5-6
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pubmed:meshHeading |
pubmed-meshheading:8789435-Base Sequence,
pubmed-meshheading:8789435-DNA, Neoplasm,
pubmed-meshheading:8789435-DNA-Binding Proteins,
pubmed-meshheading:8789435-Forkhead Transcription Factors,
pubmed-meshheading:8789435-Gene Amplification,
pubmed-meshheading:8789435-Homeodomain Proteins,
pubmed-meshheading:8789435-Humans,
pubmed-meshheading:8789435-Molecular Sequence Data,
pubmed-meshheading:8789435-Muscle Proteins,
pubmed-meshheading:8789435-Nerve Tissue Proteins,
pubmed-meshheading:8789435-PAX7 Transcription Factor,
pubmed-meshheading:8789435-Paired Box Transcription Factors,
pubmed-meshheading:8789435-RNA, Neoplasm,
pubmed-meshheading:8789435-Rhabdomyosarcoma, Alveolar,
pubmed-meshheading:8789435-Transcription Factors,
pubmed-meshheading:8789435-Translocation, Genetic
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pubmed:year |
1996
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pubmed:articleTitle |
In vivo amplification of the PAX3-FKHR and PAX7-FKHR fusion genes in alveolar rhabdomyosarcoma.
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pubmed:affiliation |
Department of Pathology, University of Pennsylvania School of Medicine, Philadelphia 19104, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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