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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1996-10-21
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pubmed:abstractText |
The effect of the fluorescent Ca2+ indicator dye Fura-2 on Ca2+ dynamics was studied in proximal apical dendrites of neocortical layer V and hippocampal CA1 pyramidal neurons in rat brain slices using somatic whole-cell recording and a charge-coupled device camera. A single action potential evoked a transient increase of intradendritic calcium concentration ([Ca2+]i) that was reduced in size and prolonged when the Fura-2 concentration was increased from 20 to 250 microM. Extrapolation to zero Fura-2 concentration suggests that "physiological" transients at 37 degrees C have large amplitudes (150-300 nM) and fast decays (time constant < 100 ms). Assuming a homogeneous compartment model for the dendrite, 0.5-1% of the total Ca2+ entering during an action potential was estimated to remain free. Washout of cytoplasmic Ca2+ buffers was not detectable, suggesting that they are relatively immobile. During trains of action potentials, [Ca2+]i increased and rapidly reached a steady state (time constant < 200 ms), fluctuating around a plateau level which depended linearly on the action potential frequency. Thus, the mean dendritic [Ca2+]i encodes the action potential frequency during physiological patterns of electrical activity and may regulate Ca(2+)-dependent dendritic functions in an activity-dependent way.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-1318182,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-1331424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-1350327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-1359030,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-1361128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-1384200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-1420931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-1459228,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-1648936,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-1906783,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-2062120,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-2169591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-2317553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-2451806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-2479140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-2571090,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-3803567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-3838314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-6200352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-7472352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-7473260,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-7512352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-7525892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-7536248,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-7544443,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-7580160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-7580163,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-7658365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-7666160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-7716524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-7791901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-7834745,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-7932239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-8046445,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-8107777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-8201402,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-8271200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-8271224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-8301352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-8351200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-8421494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-8431551,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8789126-8606791
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0006-3495
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
70
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1069-81
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8789126-Action Potentials,
pubmed-meshheading:8789126-Animals,
pubmed-meshheading:8789126-Biophysical Phenomena,
pubmed-meshheading:8789126-Biophysics,
pubmed-meshheading:8789126-Buffers,
pubmed-meshheading:8789126-Calcium,
pubmed-meshheading:8789126-Cell Compartmentation,
pubmed-meshheading:8789126-Dendrites,
pubmed-meshheading:8789126-Fluorescent Dyes,
pubmed-meshheading:8789126-Fura-2,
pubmed-meshheading:8789126-Indicators and Reagents,
pubmed-meshheading:8789126-Kinetics,
pubmed-meshheading:8789126-Models, Neurological,
pubmed-meshheading:8789126-Pyramidal Cells,
pubmed-meshheading:8789126-Rats,
pubmed-meshheading:8789126-Rats, Wistar,
pubmed-meshheading:8789126-Signal Transduction
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pubmed:year |
1996
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pubmed:articleTitle |
Ca2+ buffering and action potential-evoked Ca2+ signaling in dendrites of pyramidal neurons.
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pubmed:affiliation |
Abteilung Zellphysiologie, Max-Planck-Institut für medizinische Forschung, Heidelberg, Germany.
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pubmed:publicationType |
Journal Article,
In Vitro
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