Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1996-10-21
pubmed:abstractText
A sensitive method based on competitive PCR was developed to detect and quantitate enteroviral RNA in clinical specimens, with special emphasis on controlling contamination and the presence of potential inhibitory factors in the specimens. Oligonucleotide primers from the conserved parts of the 5' untranslated and VP2 capsid protein-coding regions were selected to differentiate between enteroviruses and rhinoviruses on the basis of the length of the cDNA amplicons. RNA transcribed from a truncated cDNA copy of the echovirus 11 genome was used as an internal control for the reverse transcription reaction and PCR. This allowed simple differentiation of the control and viral PCR products from each other by agarose gel electrophoresis and nonradioactive quantitation of the viral RNA in the clinical specimens. By direct sequencing of the PCR products and subsequent computer analysis of the data, potential laboratory contaminations could be monitored and enteroviruses from clinical samples could be grouped into four distinct clusters, thus enabling genetic typing of the viruses. The described method can be applied to the diagnosis and epidemiology of enteroviral infections.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8789006-1370845, http://linkedlifedata.com/resource/pubmed/commentcorrection/8789006-1400493, http://linkedlifedata.com/resource/pubmed/commentcorrection/8789006-1528901, http://linkedlifedata.com/resource/pubmed/commentcorrection/8789006-1716072, http://linkedlifedata.com/resource/pubmed/commentcorrection/8789006-1979752, http://linkedlifedata.com/resource/pubmed/commentcorrection/8789006-2157735, http://linkedlifedata.com/resource/pubmed/commentcorrection/8789006-2161866, http://linkedlifedata.com/resource/pubmed/commentcorrection/8789006-2170576, http://linkedlifedata.com/resource/pubmed/commentcorrection/8789006-2181447, http://linkedlifedata.com/resource/pubmed/commentcorrection/8789006-2481313, http://linkedlifedata.com/resource/pubmed/commentcorrection/8789006-2544679, http://linkedlifedata.com/resource/pubmed/commentcorrection/8789006-2558157, http://linkedlifedata.com/resource/pubmed/commentcorrection/8789006-271968, http://linkedlifedata.com/resource/pubmed/commentcorrection/8789006-2826669, http://linkedlifedata.com/resource/pubmed/commentcorrection/8789006-2838971, http://linkedlifedata.com/resource/pubmed/commentcorrection/8789006-6086949, http://linkedlifedata.com/resource/pubmed/commentcorrection/8789006-6097599, http://linkedlifedata.com/resource/pubmed/commentcorrection/8789006-6325492, http://linkedlifedata.com/resource/pubmed/commentcorrection/8789006-6546423, http://linkedlifedata.com/resource/pubmed/commentcorrection/8789006-7650164, http://linkedlifedata.com/resource/pubmed/commentcorrection/8789006-7676647, http://linkedlifedata.com/resource/pubmed/commentcorrection/8789006-7679404, http://linkedlifedata.com/resource/pubmed/commentcorrection/8789006-7691879, http://linkedlifedata.com/resource/pubmed/commentcorrection/8789006-7751371, http://linkedlifedata.com/resource/pubmed/commentcorrection/8789006-7762294, http://linkedlifedata.com/resource/pubmed/commentcorrection/8789006-7966616, http://linkedlifedata.com/resource/pubmed/commentcorrection/8789006-8030260, http://linkedlifedata.com/resource/pubmed/commentcorrection/8789006-8150936, http://linkedlifedata.com/resource/pubmed/commentcorrection/8789006-8253959, http://linkedlifedata.com/resource/pubmed/commentcorrection/8789006-8380179, http://linkedlifedata.com/resource/pubmed/commentcorrection/8789006-8380182, http://linkedlifedata.com/resource/pubmed/commentcorrection/8789006-8388893
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
34
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
313-8
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Identification of enteroviruses in clinical specimens by competitive PCR followed by genetic typing using sequence analysis.
pubmed:affiliation
Department of Virology, University of Turku, Finland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't