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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1996-9-20
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pubmed:abstractText |
IL-15 is a newly identified cytokine that has T cell and B cell growth factor activity similar to that of IL-2. In this study, a novel biologic function of IL-15 to promote cytokine production by human Th cells has been elucidated. Dermatophagoides farinae 11 (a major allergen of house dust mite)-specific human T cell clones produced IL-5 in response to recombinant human IL-15 as well as to either anti-CD3 or IL-2 stimulation. IL-5 mRNA became detectable 3 h after IL-15 stimulation and reached a maximum at 9 h. Human IL-5 promoter/enhancer-luciferase gene construct transfected to T cell clones was clearly transcribed in response to IL-15, indicating that the approximately 500-bp human IL-5 promoter/enhancer segment 5' upstream of the coding region sufficiently responded to IL-15. IL-15-induced IL-5 synthesis was completely inhibited by the tyrosine kinase inhibitor, herbimycin A, suggesting the involvement of tyrosine kinases in the signal transduction leading to IL-5 synthesis as well as to proliferation of T cells induced by IL-15. Whereas IL-5 production by human peripheral T cells was abolished by the addition of anti-IL-2-neutralizing Abs into the culture, IL-15 restored the IL-5 synthesis despite effective IL-2 neutralization. IL-15 produced at the site of allergic inflammation may play a role in the recruitment and activation of eosinophils by inducing IL-5 (a Th2 cytokine) production by T cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Actins,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Benzoquinones,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-15,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-5,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukins,
http://linkedlifedata.com/resource/pubmed/chemical/Lactams, Macrocyclic,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Quinones,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/herbimycin
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0022-1767
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pubmed:author |
pubmed-author:AsakuraYY,
pubmed-author:HoshinoAA,
pubmed-author:InoueSS,
pubmed-author:ItoKK,
pubmed-author:KaminumaOO,
pubmed-author:MoriAA,
pubmed-author:NagahoriTT,
pubmed-author:NishizakiYY,
pubmed-author:OhmuraTT,
pubmed-author:OkudairaHH,
pubmed-author:OkumuraYY,
pubmed-author:SatoGG,
pubmed-author:SulcRR
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
156
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2400-5
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:8786297-Actins,
pubmed-meshheading:8786297-Antibodies,
pubmed-meshheading:8786297-Base Sequence,
pubmed-meshheading:8786297-Benzoquinones,
pubmed-meshheading:8786297-Clone Cells,
pubmed-meshheading:8786297-DNA Primers,
pubmed-meshheading:8786297-Enhancer Elements, Genetic,
pubmed-meshheading:8786297-Enzyme Inhibitors,
pubmed-meshheading:8786297-Gene Expression,
pubmed-meshheading:8786297-Humans,
pubmed-meshheading:8786297-Interleukin-15,
pubmed-meshheading:8786297-Interleukin-2,
pubmed-meshheading:8786297-Interleukin-5,
pubmed-meshheading:8786297-Interleukins,
pubmed-meshheading:8786297-Lactams, Macrocyclic,
pubmed-meshheading:8786297-Molecular Sequence Data,
pubmed-meshheading:8786297-Plasmids,
pubmed-meshheading:8786297-Promoter Regions, Genetic,
pubmed-meshheading:8786297-Protein-Tyrosine Kinases,
pubmed-meshheading:8786297-Quinones,
pubmed-meshheading:8786297-Receptors, Interleukin-2,
pubmed-meshheading:8786297-Recombinant Proteins,
pubmed-meshheading:8786297-T-Lymphocytes, Helper-Inducer,
pubmed-meshheading:8786297-Transfection
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pubmed:year |
1996
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pubmed:articleTitle |
IL-15 promotes cytokine production of human T helper cells.
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pubmed:affiliation |
Departments of Medicine, Faculty of Medicine, University of Tokyo, Japan.
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pubmed:publicationType |
Journal Article
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