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rdf:type |
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lifeskim:mentions |
umls-concept:C0013203,
umls-concept:C0019704,
umls-concept:C0023689,
umls-concept:C0026882,
umls-concept:C0032520,
umls-concept:C0042762,
umls-concept:C0443286,
umls-concept:C1318970,
umls-concept:C1511790,
umls-concept:C1707455,
umls-concept:C1883562,
umls-concept:C2699414
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pubmed:issue |
7
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pubmed:dateCreated |
1996-12-9
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pubmed:abstractText |
Sequence-specific PCR was used in six laboratories and a ligase detection reaction was used in one laboratory to detect the zidovudine-resistance mutation at codon 215 of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase DNA. The genotypes of 27 different clinical samples, including cultured HIV-1 isolates, peripheral blood mononuclear cells, and plasma, were correctly identified by 140 of 154 (91%) assays. The sensitivity for detecting a mutation was 96% for HIV-1 reverse transcriptase DNA clone mixtures containing 30% mutant DNA and 62% for mixtures containing 6% mutant DNA.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8784610-1370174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8784610-1383414,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8784610-1709563,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8784610-1716788,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8784610-1955709,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8784610-7509000,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8784610-7512829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8784610-7538548,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8784610-7539472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8784610-7680058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8784610-7714190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8784610-7856987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8784610-8133086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8784610-8173508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8784610-8328771,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8784610-8495407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8784610-8567926,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8784610-8573378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8784610-8819586
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0095-1137
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pubmed:author |
pubmed-author:DimitrovDD,
pubmed-author:EriceAA,
pubmed-author:FrenkelL MLM,
pubmed-author:IversenAA,
pubmed-author:JapourA JAJ,
pubmed-author:KuritzkesD RDR,
pubmed-author:MayersD LDL,
pubmed-author:PenaFF,
pubmed-author:ReichelderferP SPS,
pubmed-author:SannerudK JKJ,
pubmed-author:ShaferR WRW,
pubmed-author:WeislowO SOS,
pubmed-author:WhiteFF,
pubmed-author:WintersM AMA
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pubmed:issnType |
Print
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pubmed:volume |
34
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1849-53
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8784610-Antiviral Agents,
pubmed-meshheading:8784610-Base Sequence,
pubmed-meshheading:8784610-Codon,
pubmed-meshheading:8784610-DNA, Viral,
pubmed-meshheading:8784610-DNA Ligases,
pubmed-meshheading:8784610-DNA Primers,
pubmed-meshheading:8784610-Drug Resistance, Microbial,
pubmed-meshheading:8784610-Evaluation Studies as Topic,
pubmed-meshheading:8784610-Genotype,
pubmed-meshheading:8784610-HIV Infections,
pubmed-meshheading:8784610-HIV-1,
pubmed-meshheading:8784610-Humans,
pubmed-meshheading:8784610-Laboratories,
pubmed-meshheading:8784610-Leukocytes, Mononuclear,
pubmed-meshheading:8784610-Mutation,
pubmed-meshheading:8784610-Plasma,
pubmed-meshheading:8784610-Polymerase Chain Reaction,
pubmed-meshheading:8784610-Sensitivity and Specificity,
pubmed-meshheading:8784610-Zidovudine
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pubmed:year |
1996
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pubmed:articleTitle |
Interlaboratory comparison of sequence-specific PCR and ligase detection reaction to detect a human immunodeficiency virus type 1 drug resistance mutation. The AIDS Clinical Trials Group Virology Committee Drug Resistance Working Group.
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pubmed:affiliation |
Stanford University, California, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study
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