Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
|
| pubmed:dateCreated |
1996-10-17
|
| pubmed:databankReference | |
| pubmed:abstractText |
Vasoactive intestinal peptide (VIP), a neuropeptide present in primary and secondary lymphoid organs has been previously reported to inhibit IL-2 and IL-4 production as well as the proliferation of mitogen- or antigen-stimulated T-cells. Binding studies suggested that the immunoregulatory effects of VIP are mediated through specific VIP-binding sites present on lymphocyte subpopulations. Here we report on the expression of VIP-R1 mRNA in various murine lymphocyte subpopulations. By using RT-PCR. RNase protection assay, cDNA cloning, and sequence analysis, we show that stimulated and unstimulated murine spleen cells, thymocytes. CD4+ and CD8+ T-cells express VIP-R1. The VIP-R1 fragment amplified from murine brain, thymocytes, spleen cells and CD4+ T-cells share identical nucleotide sequences, and a high degree of homology with the corresponding nonlymphoid rat and human VIP-R1 sequences. The expression of VIP-R1 in thymocytes and peripheral lymphocytes, and especially in the CD4+ T-cell subset supports the idea that VIP produced or released locally in the lymphoid microenvironment could directly affect cytokine production and proliferation of T-lymphocytes.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0165-5728
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
68
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
109-19
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:8784267-Animals,
pubmed-meshheading:8784267-Blotting, Northern,
pubmed-meshheading:8784267-Blotting, Southern,
pubmed-meshheading:8784267-Brain,
pubmed-meshheading:8784267-CD4-Positive T-Lymphocytes,
pubmed-meshheading:8784267-CD8-Positive T-Lymphocytes,
pubmed-meshheading:8784267-Female,
pubmed-meshheading:8784267-Lung,
pubmed-meshheading:8784267-Mice,
pubmed-meshheading:8784267-Mice, Inbred BALB C,
pubmed-meshheading:8784267-Molecular Sequence Data,
pubmed-meshheading:8784267-Polymerase Chain Reaction,
pubmed-meshheading:8784267-RNA, Messenger,
pubmed-meshheading:8784267-Receptors, Vasoactive Intestinal Peptide,
pubmed-meshheading:8784267-Receptors, Vasoactive Intestinal Polypeptide, Type I,
pubmed-meshheading:8784267-Sequence Analysis, DNA,
pubmed-meshheading:8784267-Sequence Homology, Nucleic Acid,
pubmed-meshheading:8784267-Spleen,
pubmed-meshheading:8784267-Thymus Gland
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Murine T-lymphocytes express vasoactive intestinal peptide receptor 1 (VIP-R1) mRNA.
|
| pubmed:affiliation |
Department of Biological Sciences, Rutgers University, New York, NJ 07102, USA.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|