Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1996-10-16
|
| pubmed:abstractText |
Bone marrow transplantation (BMT) is followed by a period of profound immune deficiency, during which new T lymphocytes are generated from either stem cells or immature thymic progenitors. Interleukin-7 (IL-7) induces proliferation and differentiation of immature thymocytes. We examined whether the in vivo administration of IL-7 to mice receiving BMT would alter thymic reconstitution. Lethally irradiated C57BL/6 mice received syngeneic BMT, followed by either IL-7 or placebo from days 5 to 18 post-BMT. At day 28, BMT recipients that had not received IL-7 had profound thymic hypoplasia (< 5% of normal), with relative increases in the numbers of immature thymocytes, decreased numbers of mature peripheral (splenic) T lymphocytes, and severely impaired T- and B-cell function. In contrast, transplanted mice treated with IL-7 had normalization of thymic cellularity, with normal proportions of thymic subsets and T-cell receptor beta variable gene (TCRV beta) usage, normal numbers of peripheral CD4+ T lymphocytes, and improved antigen-specific T- and B-cell function. In the BMT-IL-7 mice, there was an eightfold increase in the number of immature CD3-CD4-CD8- thymocytes in G2-M of the cell cycle, indicating that restoration of thymic cellularity was due to enhanced proliferation of immature thymic progenitors. Similar effects following IL-7 administration were also observed when donor bone marrow was depleted of mature T lymphocytes, indicating that IL-7 administration affected immature hematopoietic progenitors. IL-7 promotes thymic reconstitution after BMT, and may be useful in preventing post-BMT immune deficiency.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0006-4971
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
88
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1887-94
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:8781449-Animals,
pubmed-meshheading:8781449-Bone Marrow Transplantation,
pubmed-meshheading:8781449-Cell Cycle,
pubmed-meshheading:8781449-Female,
pubmed-meshheading:8781449-Gene Rearrangement, beta-Chain T-Cell Antigen Receptor,
pubmed-meshheading:8781449-Graft Survival,
pubmed-meshheading:8781449-Hematopoiesis,
pubmed-meshheading:8781449-Hematopoietic Cell Growth Factors,
pubmed-meshheading:8781449-Immunologic Deficiency Syndromes,
pubmed-meshheading:8781449-Interleukin-7,
pubmed-meshheading:8781449-Lymphocyte Activation,
pubmed-meshheading:8781449-Lymphocyte Count,
pubmed-meshheading:8781449-Male,
pubmed-meshheading:8781449-Mice,
pubmed-meshheading:8781449-Mice, Inbred C57BL,
pubmed-meshheading:8781449-Radiation Chimera,
pubmed-meshheading:8781449-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:8781449-Spleen,
pubmed-meshheading:8781449-Stimulation, Chemical,
pubmed-meshheading:8781449-T-Lymphocyte Subsets,
pubmed-meshheading:8781449-Thymus Gland
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Enhancement of thymopoiesis after bone marrow transplant by in vivo interleukin-7.
|
| pubmed:affiliation |
Department of Pediatrics, Childrens Hospital Los Angeles, University of Southern California School of Medicine, Los Angeles, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|