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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5282
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pubmed:dateCreated |
1996-10-11
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pubmed:abstractText |
Group I self-splicing introns catalyze their own excision from precursor RNAs by way of a two-step transesterification reaction. The catalytic core of these ribozymes is formed by two structural domains. The 2.8-angstrom crystal structure of one of these, the P4-P6 domain of the Tetrahymena thermophila intron, is described. In the 160-nucleotide domain, a sharp bend allows stacked helices of the conserved core to pack alongside helices of an adjacent region. Two specific long-range interactions clamp the two halves of the domain together: a two-Mg2+-coordinated adenosine-rich corkscrew plugs into the minor groove of a helix, and a GAAA hairpin loop binds to a conserved 11-nucleotide internal loop. Metal- and ribose-mediated backbone contacts further stabilize the close side-by-side helical packing. The structure indicates the extent of RNA packing required for the function of large ribozymes, the spliceosome, and the ribosome.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0036-8075
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
273
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1678-85
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8781224-Adenine,
pubmed-meshheading:8781224-Animals,
pubmed-meshheading:8781224-Base Composition,
pubmed-meshheading:8781224-Base Sequence,
pubmed-meshheading:8781224-Binding Sites,
pubmed-meshheading:8781224-Catalysis,
pubmed-meshheading:8781224-Crystallography, X-Ray,
pubmed-meshheading:8781224-Hydrogen Bonding,
pubmed-meshheading:8781224-Introns,
pubmed-meshheading:8781224-Magnesium,
pubmed-meshheading:8781224-Models, Molecular,
pubmed-meshheading:8781224-Molecular Sequence Data,
pubmed-meshheading:8781224-Nucleic Acid Conformation,
pubmed-meshheading:8781224-Phosphates,
pubmed-meshheading:8781224-Phylogeny,
pubmed-meshheading:8781224-RNA, Catalytic,
pubmed-meshheading:8781224-RNA, Protozoan,
pubmed-meshheading:8781224-RNA Splicing,
pubmed-meshheading:8781224-Ribose,
pubmed-meshheading:8781224-Tetrahymena thermophila
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pubmed:year |
1996
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pubmed:articleTitle |
Crystal structure of a group I ribozyme domain: principles of RNA packing.
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pubmed:affiliation |
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA. doudna@csb.yale.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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