Linked Life Data

8781190

Source:http://linkedlifedata.com/resource/pubmed/id/8781190

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1996-11-7
pubmed:abstractText
The time course of calcium-activated chloride "tail" currents (Itail) in single cells of the rabbit portal vein was studied. These currents were activated by the influx of calcium through voltage-dependent calcium channels (VDCCs). At -50 mV, Itail decayed exponentially with a time constant (tau) of 80-100 ms that was independent of amplitude and was similar to the tau of the decay of spontaneous transient inward currents (STICs; calcium-activated chloride currents). The decays of the STIC and Itail had a similar voltage dependence between -50 and -110 mV and were similarly affected by the chloride channel blocker, niflumic acid. However, at more positive potentials (-20 to +40 mV), Itail was sustained for the duration of the test pulse in most cells, in contrast to STICs which decayed exponentially. At very positive potentials (e.g. +100 mV), when little calcium enters the cell through VDCCs, Itail decayed exponentially. Measurement of calcium current (ICa) at various potentials showed that the VDCCs did not inactivate fully at potentials between -20 and +30 mV. We propose that at negative potentials the decay of Itail is determined by slow gating of the chloride channel, but at positive potentials a sustained Itail is produced by persistent influx of calcium through non-inactivating VDCCs.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0031-6768
pubmed:author
pubmed:issnType
Print
pubmed:volume
432
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
970-9
pubmed:dateRevised
2009-9-29
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Analysis of the time course of calcium-activated chloride "tail" currents in rabbit portal vein smooth muscle cells.
pubmed:affiliation
Department of Pharmacology and Clinical Pharmacology, St. George's Hospital Medical School, Cranmer Terrace, London SW17 0RE, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't