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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1996-9-13
|
| pubmed:abstractText |
A Nafion-loaded carbon paste electrode (CPE) was prepared and its square wave voltammetric response at a fresh surface was optimized using (ferrocenylmethyl)-trimethylammonium iodide (1) as a model of a cation that accumulates irreversibly. Low current responses could be obtained reproducibly without preconditioning at a CPE loaded with 1-octanol and neutralized Nafion. Use of the former made the method more sensitive, and use of the latter gave lower background currents and stable analytical response over a long period of Nafion-loaded CPE use. Under optimized conditions and using a 5 min accumulation period, the modified electrode gave a linear current response over the range of 5 nM to 1.5 microM 1, a detection limit of 5 nM 1 and a relative standard deviation of 5% for 0.5 microM 1. Using these optimized conditions, the competitive homogeneous immunoassay of phenytoin as a model hapten was carried out with cobaltocenium-labeled phenytoin as tracer. Clinical serum samples (5 microL) of phenytoin at therapeutic concentrations (20-80 microM) could be analyzed using this method.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0003-2700
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
68
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
930-5
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading | |
| pubmed:year |
1996
|
| pubmed:articleTitle |
Renewable perfluorosulfonated ionomer carbon paste electrode for competitive homogeneous electrochemical immunoassays using a redox cationic labeled hapten.
|
| pubmed:affiliation |
Laboratoire d'Electrochimie Organique, Université Blaise Pascal de Clermont-Ferrand, Aubière, France.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|