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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1996-10-21
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pubmed:databankReference |
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pubmed:abstractText |
Using conserved fingerprints in the glycosyltransferase (GTase) domain of high-molecular-weight penicillin-binding proteins (PBP), a gene (mgt) encoding a putative monofunctional glycosyltransferase has been identified in Haemophilus influenzae and in other bacteria] species. Here we report the cloning of the homologous Escherichia coli gene and show that the solubilised membrane fraction of E. coli cells overexpressing the mgt gene contain a significantly increased peptidoglycan synthesis activity. In contrast to the high-molecular-weight PBPs, this activity is not inhibited by Flavomycin.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
26
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pubmed:volume |
392
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
184-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8772200-Amino Acid Sequence,
pubmed-meshheading:8772200-Base Sequence,
pubmed-meshheading:8772200-DNA Primers,
pubmed-meshheading:8772200-Escherichia coli,
pubmed-meshheading:8772200-Glycosyltransferases,
pubmed-meshheading:8772200-Membrane Proteins,
pubmed-meshheading:8772200-Molecular Sequence Data,
pubmed-meshheading:8772200-Peptidoglycan,
pubmed-meshheading:8772200-Recombinant Proteins,
pubmed-meshheading:8772200-Sequence Homology, Amino Acid,
pubmed-meshheading:8772200-Species Specificity
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pubmed:year |
1996
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pubmed:articleTitle |
The monofunctional glycosyltransferase of Escherichia coli is a member of a new class of peptidoglycan-synthesising enzymes.
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pubmed:affiliation |
Pharma Research Department, F. Hoffman-La Roche Ltd., Basel, Switzerland.
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pubmed:publicationType |
Journal Article
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