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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1996-10-10
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pubmed:abstractText |
A type III iodothyronine deiodinase (D-III) that inactivates thyroid hormones has been recently cloned and identified as a selenoprotein in neonatal rat skin. However, selenium (Se) deficiency does not affect the D-III activity in the rat placenta and decreases the D-III in the rat brain only slightly. This study examines the effect of Se on the D-III activity in cultures of rat brain astrocytes. Astrocytes were depleted in Se by maintaining them in Se-free chemically defined medium for 7 days. These conditions decreased the activity of a recognized selenoprotein, glutathione peroxidase, 3-10-fold. D-III activity induced by 12-0-tetradecanoylphorbol-13-acetate (TPA) was also decreased 2-6-fold. Addition of 30 nM Se to the culture medium caused a rapid increase in TPA-induced D-III activity visible within 1 h. This Se effect was maximal at 3 h (4-fold increase) and dose-dependent. Se also increased the induction of D-III by acidic Fibroblast Growth Factor, 8-bromo-cAMP, T4, or retinoic acid. Cycloheximide blocked the effect of Se on TPA-induced D-III activity, whereas actinomycin D did not. Thus the rapid effect of Se does not require messenger RNA synthesis but requires protein synthesis. We conclude that the D-III in astrocytes is probably a selenoprotein.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/8-Bromo Cyclic Adenosine...,
http://linkedlifedata.com/resource/pubmed/chemical/Cycloheximide,
http://linkedlifedata.com/resource/pubmed/chemical/Dactinomycin,
http://linkedlifedata.com/resource/pubmed/chemical/Fibroblast Growth Factor 1,
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione Peroxidase,
http://linkedlifedata.com/resource/pubmed/chemical/Iodide Peroxidase,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Selenium,
http://linkedlifedata.com/resource/pubmed/chemical/Selenoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate,
http://linkedlifedata.com/resource/pubmed/chemical/Thyroxine,
http://linkedlifedata.com/resource/pubmed/chemical/Tretinoin
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0013-7227
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
137
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3021-5
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:8770927-8-Bromo Cyclic Adenosine Monophosphate,
pubmed-meshheading:8770927-Animals,
pubmed-meshheading:8770927-Animals, Newborn,
pubmed-meshheading:8770927-Astrocytes,
pubmed-meshheading:8770927-Brain,
pubmed-meshheading:8770927-Cells, Cultured,
pubmed-meshheading:8770927-Cycloheximide,
pubmed-meshheading:8770927-Dactinomycin,
pubmed-meshheading:8770927-Female,
pubmed-meshheading:8770927-Fibroblast Growth Factor 1,
pubmed-meshheading:8770927-Glutathione Peroxidase,
pubmed-meshheading:8770927-Iodide Peroxidase,
pubmed-meshheading:8770927-Isoenzymes,
pubmed-meshheading:8770927-Kinetics,
pubmed-meshheading:8770927-Placenta,
pubmed-meshheading:8770927-Pregnancy,
pubmed-meshheading:8770927-Protein Biosynthesis,
pubmed-meshheading:8770927-Proteins,
pubmed-meshheading:8770927-Rats,
pubmed-meshheading:8770927-Rats, Sprague-Dawley,
pubmed-meshheading:8770927-Selenium,
pubmed-meshheading:8770927-Selenoproteins,
pubmed-meshheading:8770927-Tetradecanoylphorbol Acetate,
pubmed-meshheading:8770927-Thyroxine,
pubmed-meshheading:8770927-Tretinoin
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pubmed:year |
1996
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pubmed:articleTitle |
Evidence that type III iodothyronine deiodinase in rat astrocyte is a selenoprotein.
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pubmed:affiliation |
U. 96 INSERM - Unité de Recherche sur la Glande Thyroïde et la Régulation Hormonale, Le Kremlin-Bicêtre Cedex, France.
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pubmed:publicationType |
Journal Article
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