Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1996-10-23
|
| pubmed:abstractText |
In order to establish whether specific MHC class II-peptide complexes are capable of selecting TCR V regions, we investigated in detail the TCR beta chain used in the recognition of HLA-DR3 restricted hsp65 peptide 3-13 in a tuberculoid leprosy patient. Using RT-PCR, a clear dominance of the TCRBV5 gene family was observed in a hsp65 peptide 3-13-specific T-cell line; however, not in fresh, unstimulated PBMCs, PHA-stimulated PBMCs, or a T-cell line specific for tetanus toxoid. DNA sequence analysis of the TCR V regions, comprising TCRBV5 genes, derived from the hsp65 peptide 3-13-specific T-cell line revealed the exclusive usage of the TCRBV55S1 gene segment and a predominance of one V-D-J gene rearrangement, which is indicative of clonal expansion of these T lymphocytes. Additional highly similar V-D-J gene rearrangements were detected at a low level in this hsp65 peptide 3-13 specific T-cell line. These conserved junctional regions (CDR3 regions) could not be detected within the TCRBV5 gene family of fresh PBMCs, PHA-stimulated PBMCs, hsp65, and tetanus-toxoid-specific T-cell lines from this patient. The observations in this tuberculoid leprosy patient reveal that an HLA class-II-restricted T-cell response results in selection of TCRBV regions which are highly similar in amino acid composition to the CDR3 region within the expanding TCRBV regions.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Chaperonin 60,
http://linkedlifedata.com/resource/pubmed/chemical/Chaperonins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DR3 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Variable Region,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...,
http://linkedlifedata.com/resource/pubmed/chemical/heat-shock protein 65, Mycobacterium
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0198-8859
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
44
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
220-7
|
| pubmed:dateRevised |
2009-11-19
|
| pubmed:meshHeading |
pubmed-meshheading:8770635-Amino Acid Sequence,
pubmed-meshheading:8770635-Antigens, Bacterial,
pubmed-meshheading:8770635-Bacterial Proteins,
pubmed-meshheading:8770635-Base Sequence,
pubmed-meshheading:8770635-Cell Line,
pubmed-meshheading:8770635-Chaperonin 60,
pubmed-meshheading:8770635-Chaperonins,
pubmed-meshheading:8770635-Conserved Sequence,
pubmed-meshheading:8770635-DNA Primers,
pubmed-meshheading:8770635-HLA-DR3 Antigen,
pubmed-meshheading:8770635-Humans,
pubmed-meshheading:8770635-Immunoglobulin Variable Region,
pubmed-meshheading:8770635-Leprosy, Tuberculoid,
pubmed-meshheading:8770635-Molecular Sequence Data,
pubmed-meshheading:8770635-Mycobacterium leprae,
pubmed-meshheading:8770635-Peptide Fragments,
pubmed-meshheading:8770635-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:8770635-T-Lymphocytes
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Clonal dominance and selection for similar complementarity determining region 3 motifs among T lymphocytes responding to the HLA-DR3-associated Mycobacterium leprae heat shock protein 65-kd peptide 3-13.
|
| pubmed:affiliation |
Department of Immunohematology and Blood Bank, University Hospital Leiden, The Netherlands.
|
| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
|