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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2 Pt 1
|
| pubmed:dateCreated |
1996-12-5
|
| pubmed:abstractText |
The relationship between 3-O-methyl-D-glucose transport and 2-N-4-(1-azi-2,2,2-trifluoroethyl)-benzoyl-1, 3-bis-(D-mannos-4-yloxy)-2-propylamine (ATB-BMPA)-labeled cell surface GLUT-4 protein was assessed in fast-twitch (epitrochlearis) and slow-twitch (soleus) muscles of lean and obese (fa/fa) Zucker rats. In the absence of insulin, glucose transport as well as cell surface GLUT-4 protein was similar in both epitrochlearis and soleus muscles of lean and obese rats. In contrast, insulin-stimulated glucose transport rates were significantly higher for lean than obese rats in both soleus (0.74 +/- 0.05 vs. 0.40 +/- 0.02 mumol.g-1.10 min-1) and epitrochlearis (0.51 +/- 0.05 vs. 0.17 +/- 0.02 mumol.g-1.10 min-1) muscles. The ability of insulin to enhance glucose transport in fast- and slow-twitch muscles from both lean and obese rats corresponded directly with changes in cell surface GLUT-4 protein. Muscle contraction elicited similar increases in glucose transport in lean and obese rats, with the effect being more pronounced in fast-twitch (0.70 +/- 0.07 and 0.77 +/- 0.04 mumol.g-1.10 min-1 for obese and lean, respectively) than in slow-twitch muscle (0.36 +/- 0.03 and 0.40 +/- 0.02 mumol.g-1.10 min-1 for obese and lean, respectively). The contraction-induced changes in glucose transport directly corresponded with the observed changes in cell surface GLUT-4 protein. Thus the reduced glucose transport response to insulin in skeletal muscle of the obese Zucker rat appears to result directly from an inability to effectively enhance cell surface GLUT-4 protein.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2-N-(4-(1-azitrifluoroethyl)benzoyl)...,
http://linkedlifedata.com/resource/pubmed/chemical/Affinity Labels,
http://linkedlifedata.com/resource/pubmed/chemical/Azides,
http://linkedlifedata.com/resource/pubmed/chemical/Disaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose Transporter Type 4,
http://linkedlifedata.com/resource/pubmed/chemical/Glycogen,
http://linkedlifedata.com/resource/pubmed/chemical/Glycosides,
http://linkedlifedata.com/resource/pubmed/chemical/Monosaccharide Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Muscle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Propylamines,
http://linkedlifedata.com/resource/pubmed/chemical/Slc2a4 protein, rat
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0002-9513
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
271
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
E294-301
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8770023-Affinity Labels,
pubmed-meshheading:8770023-Animals,
pubmed-meshheading:8770023-Azides,
pubmed-meshheading:8770023-Biological Transport,
pubmed-meshheading:8770023-Cell Membrane,
pubmed-meshheading:8770023-Disaccharides,
pubmed-meshheading:8770023-Extremities,
pubmed-meshheading:8770023-Female,
pubmed-meshheading:8770023-Glucose,
pubmed-meshheading:8770023-Glucose Transporter Type 4,
pubmed-meshheading:8770023-Glycogen,
pubmed-meshheading:8770023-Glycosides,
pubmed-meshheading:8770023-Monosaccharide Transport Proteins,
pubmed-meshheading:8770023-Muscle, Skeletal,
pubmed-meshheading:8770023-Muscle Contraction,
pubmed-meshheading:8770023-Muscle Proteins,
pubmed-meshheading:8770023-Obesity,
pubmed-meshheading:8770023-Propylamines,
pubmed-meshheading:8770023-Rats,
pubmed-meshheading:8770023-Rats, Zucker
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Glucose transport and cell surface GLUT-4 protein in skeletal muscle of the obese Zucker rat.
|
| pubmed:affiliation |
Department of Kinesiology, University of Texas at Austin 78712, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|