Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1997-1-7
pubmed:abstractText
Under typical culture conditions, cerebellar granule cells die abruptly after 17 days in vitro. This burst of neuronal death involves ultrastructural changes and internucleosomal DNA fragmentations characteristic of apoptosis and is effectively arrested by pretreatment with actinomycin-D and cycloheximide. The level of a 38-kDa protein in the particulate fraction is markedly increased during age-induced cell death and by pretreatment with NMDA, which potentiates this cell death. Conversely, the age-induced increment of the 38-kDa particulate protein is suppressed by actinomycin-D and cycloheximide. N-terminal microsequencing of the 38-kDa protein revealed sequence identity with glyceraldehyde-3-phosphate dehydrogenase (GAPDH). A GAPDH antisense oligodeoxyribonucleotide blocks age-induced expression of the particulate 38-kDa protein and effectively inhibits neuronal apoptosis. In contrast, the corresponding sense oligonucleotide of GAPDH was completely ineffective in preventing the age-induced neuronal death and the 38-kDa protein overexpression. Moreover, the age-induced expression of the 38-kDa protein is preceded by a pronounced increase in the GAPDH mRNA level, which is abolished by actinomycin-D, cycloheximide, or the GAPDH antisense, but not sense, oligonucleotide. Thus, our results suggest that overexpression of GAPDH in the particulate fraction has a direct role in age-induced apoptosis of cerebellar neurons.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-3042
pubmed:author
pubmed:issnType
Print
pubmed:volume
66
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
928-35
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed-meshheading:8769851-Aging, pubmed-meshheading:8769851-Amino Acid Sequence, pubmed-meshheading:8769851-Animals, pubmed-meshheading:8769851-Apoptosis, pubmed-meshheading:8769851-Base Sequence, pubmed-meshheading:8769851-Cell Aging, pubmed-meshheading:8769851-Cells, Cultured, pubmed-meshheading:8769851-Cerebellum, pubmed-meshheading:8769851-Cycloheximide, pubmed-meshheading:8769851-Dactinomycin, pubmed-meshheading:8769851-Glyceraldehyde-3-Phosphate Dehydrogenases, pubmed-meshheading:8769851-Molecular Sequence Data, pubmed-meshheading:8769851-N-Methylaspartate, pubmed-meshheading:8769851-Neurons, pubmed-meshheading:8769851-Neuroprotective Agents, pubmed-meshheading:8769851-Oligonucleotide Probes, pubmed-meshheading:8769851-Oligonucleotides, Antisense, pubmed-meshheading:8769851-RNA, Messenger, pubmed-meshheading:8769851-Rats, pubmed-meshheading:8769851-Rats, Sprague-Dawley
pubmed:year
1996
pubmed:articleTitle
Evidence that glyceraldehyde-3-phosphate dehydrogenase is involved in age-induced apoptosis in mature cerebellar neurons in culture.
pubmed:affiliation
Group on Cellular Neurobiology, Josai University, Saitama, Japan.
pubmed:publicationType
Journal Article