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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1996-11-6
|
| pubmed:abstractText |
Reocclusion following thrombolysis is a major limitation of thrombolytic therapy with recombinant tissue-type plasminogen activator (rt-PA) because denuded vessel wall exposed to blood following thrombolysis is a favourable surface for platelet and leucocyte deposition. We have applied a chemiluminescence technique to detect superoxide radical (0(-2)) produced by leucocytes adherent to the femoral artery 24 h after photochemically induced thrombogenesis in the guinea pig in vivo and subsequent thrombolysis by rt-PA. Intravenous administration of MCLA, a specific chemiluminescence reagent for detecting O(-2), markedly increased photon emission. the photon emission was markedly potentiated by phorbol myristate acetate and was suppressed by superoxide dismutase. Reocclusion 24 h after rt-PA induced thrombolysis was observed in 10 of 16 animals. Histological observations revealed extensive polymorphonuclear leucocytes adherent to the vessel wall at the site of thrombogenesis and thrombolysis. A higher level of 0(-2) could be detected from the arteries in which thrombolysis was induced compared with those without thrombolysis. Further, the level 0(-2) detected was greater in reoccluded arteries compared with those in which reflow was established. These observations suggest that 0(-2) is produced by adherent leucocytes at the site of thrombolysis and that leucocytes are involved in reocclusion after thrombolysis.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2-methyl-6-(4-methoxyphenyl)-3,7-dih...,
http://linkedlifedata.com/resource/pubmed/chemical/Imidazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Plasminogen Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrazines,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Rose Bengal,
http://linkedlifedata.com/resource/pubmed/chemical/Superoxides
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0021-9150
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
122
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
217-24
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:8769684-Animals,
pubmed-meshheading:8769684-Cell Adhesion,
pubmed-meshheading:8769684-Disease Models, Animal,
pubmed-meshheading:8769684-Endothelium, Vascular,
pubmed-meshheading:8769684-Femoral Artery,
pubmed-meshheading:8769684-Guinea Pigs,
pubmed-meshheading:8769684-Imidazoles,
pubmed-meshheading:8769684-Light,
pubmed-meshheading:8769684-Luminescent Measurements,
pubmed-meshheading:8769684-Male,
pubmed-meshheading:8769684-Neutrophils,
pubmed-meshheading:8769684-Plasminogen Activators,
pubmed-meshheading:8769684-Pyrazines,
pubmed-meshheading:8769684-Recombinant Proteins,
pubmed-meshheading:8769684-Rose Bengal,
pubmed-meshheading:8769684-Superoxides,
pubmed-meshheading:8769684-Thrombolytic Therapy,
pubmed-meshheading:8769684-Thrombosis
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
A chemiluminescent detection of superoxide radical produced by adherent leucocytes to the subendothelium following thrombolysis: studies with a photochemically induced thrombosis model in the guinea pig femoral artery.
|
| pubmed:affiliation |
Department of Pharmacology, Hamamatsu University School of Medicine, Japan.
|
| pubmed:publicationType |
Journal Article
|