Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
|
| pubmed:dateCreated |
1996-10-8
|
| pubmed:databankReference | |
| pubmed:abstractText |
Mojave toxin is a neurotoxic, heterodimeric phospholipase A2 (PLA2) from the venom of the Mojave rattlesnake (Crotalus scutulatus scutulatus) and is characteristic of all rattlesnake presynaptic neurotoxins. Here, we describe a phospholipase A2 pseudogene (psi-Mtx) located 2,000 nucleotides upstream, and on the opposite DNA strand, from a gene for Mojave toxin acidic subunit (Mtx-a). The pseudogene lacks the first exon and a few segments of noncoding DNA found in functional snake venom PLA2 genes, but does have the coding information for a complete PLA2 protein. psi-Mtx retains the unusual gene sequence similarity pattern found in functional viperid PLA2 genes. When compared to genes from C. s. scutulatus and the Hahn snake (Trimeresurus flavoviridus), psi-Mtx shows strong conservation of nocoding regions and variable protein-coding regions. Although the nocoding regions of psi-Mtx are conserved with respect to other viperid PLA2 genes, the three exons code for a unique PLA2-like protein similar in sequence to ammodytoxin b found in the venom of the western sand viper (Vipera ammodytes ammodytes). The structure of these genes suggests a common ancestor for all viperid PLA2 genes. Phylogenetic analysis of psi-Mtx, Mtx-a, Mtx-b, pgPLA 1a, and pgPLA 1b suggest that psi-Mtx diverged from an ancestral sequence before the presumed gene duplication event leading to Mtx-a and Mtx-b. However, analysis of the basis of coding regions alone gives a conflicting result.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Crotalid Venoms,
http://linkedlifedata.com/resource/pubmed/chemical/Mojave toxin,
http://linkedlifedata.com/resource/pubmed/chemical/Neurotoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipases A,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipases A2
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
1044-5498
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
15
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
661-8
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8769568-Amino Acid Sequence,
pubmed-meshheading:8769568-Animals,
pubmed-meshheading:8769568-Base Sequence,
pubmed-meshheading:8769568-Cloning, Molecular,
pubmed-meshheading:8769568-Conserved Sequence,
pubmed-meshheading:8769568-Crotalid Venoms,
pubmed-meshheading:8769568-Crotalus,
pubmed-meshheading:8769568-Exons,
pubmed-meshheading:8769568-Introns,
pubmed-meshheading:8769568-Molecular Sequence Data,
pubmed-meshheading:8769568-Neurotoxins,
pubmed-meshheading:8769568-Phospholipases A,
pubmed-meshheading:8769568-Phospholipases A2,
pubmed-meshheading:8769568-Phylogeny,
pubmed-meshheading:8769568-Pseudogenes,
pubmed-meshheading:8769568-Restriction Mapping,
pubmed-meshheading:8769568-Sequence Analysis, DNA,
pubmed-meshheading:8769568-Sequence Homology, Amino Acid,
pubmed-meshheading:8769568-Sequence Homology, Nucleic Acid,
pubmed-meshheading:8769568-Viperidae
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
A phospholipase A2-like pseudogene retaining the highly conserved introns of Mojave toxin and other snake venom group II PLA2s, but having different exons.
|
| pubmed:affiliation |
University of Wyoming, Department of Molecular Biology, Laramie 82071-3944, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
|