Linked Life Data

8767741

Source:http://linkedlifedata.com/resource/pubmed/id/8767741

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1996-9-16
pubmed:abstractText
This paper describes the recent history and development of capillary isoelectric focusing (cIEF), as it has evolved over the past 10 years forming a distinct mode of high-performance capillary electrophoresis (HPCE). The theory, equations, fundamentals and basics of cIEF are discussed and described, including modes of focusing and mobilization, coated vs uncoated capillaries, different detection schemes, resolutions possible, peak capacity possible and final commercialized approaches now available. Then, the applications of the technique are emphasized, as applied to smaller peptides, larger proteins and still larger antibodies and antibody-protein complexes. The emphasis has been on the application of capillary electromigration techniques in drug analysis. Throughout, attempts have been made to emphasize the potential applications and uses of cIEF methods, and how these might be successfully utilized in drug analysis and assays for larger biopolymers.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0021-9673
pubmed:author
pubmed:issnType
Print
pubmed:day
31
pubmed:volume
735
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
165-90
pubmed:dateRevised
2009-1-15
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Capillary isoelectric focusing as a tool in the examination of antibodies, peptides and proteins of pharmaceutical interest.
pubmed:affiliation
Department of Chemistry, Northeastern University, Boston, MA 02115, USA.
pubmed:publicationType
Journal Article, Review, Research Support, Non-U.S. Gov't