Linked Life Data

8765750

Source:http://linkedlifedata.com/resource/pubmed/id/8765750

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1996-9-25
pubmed:databankReference
pubmed:abstractText
We isolated and characterized genomic and cDNA clones encoding mouse senescence marker protein-30 (SMP30), the protein amounts of which are known to decrease with aging in the livers of rats. This decrease in the expression of SMP30 is independent of androgen. SMP30 is a calcium binding protein also called regucalcin. The expression of SMP30 in aged mouse liver and 5' flanking sequence of the genome were also characterized. The cDNA contained an open reading frame encoding 299 amino acids with a calculated molecular weight of 33-404. The amino acid sequence of mouse SMP30 showed 94% similarity to rat SMP30 and 89% to human SMP30. Northern blot analysis specifically detected mouse SMP30 transcript in the liver and also confirmed its significant decrease with aging. Analysis of the murine genomic clone revealed that SMP30 was organized by seven exons and six introns, spanning approx. 17.5 kb. Primer extension analysis revealed that two major transcription initiation sites were localized at 101 bp and 102 bp upstream from ATG translation initiation codon. The nucleotide (nt) sequence of 5' flanking region showed a TATA-like sequence, a CAAT box, and SP-1 sites at nt -29, -72 and -169 in the promoter region, respectively. Interestingly, we found two classes of C/EBP sites which are highly and constantly expressed in the liver, in addition to AP-2, AP-1, GATA-1, AP-1/GRE and GAGA sites. These results provide important clues for understanding the regulatory mechanism of SMP30 gene expression and its relationship to aging.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
31
pubmed:volume
1308
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
49-57
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:8765750-Aging, pubmed-meshheading:8765750-Amino Acid Sequence, pubmed-meshheading:8765750-Animals, pubmed-meshheading:8765750-Base Sequence, pubmed-meshheading:8765750-Biological Markers, pubmed-meshheading:8765750-Calcium-Binding Proteins, pubmed-meshheading:8765750-DNA, Complementary, pubmed-meshheading:8765750-Exons, pubmed-meshheading:8765750-Genomic Library, pubmed-meshheading:8765750-Intracellular Signaling Peptides and Proteins, pubmed-meshheading:8765750-Introns, pubmed-meshheading:8765750-Liver, pubmed-meshheading:8765750-Mice, pubmed-meshheading:8765750-Mice, Inbred C57BL, pubmed-meshheading:8765750-Molecular Sequence Data, pubmed-meshheading:8765750-Regulatory Sequences, Nucleic Acid, pubmed-meshheading:8765750-Sequence Homology, Amino Acid, pubmed-meshheading:8765750-Species Specificity, pubmed-meshheading:8765750-Sulfotransferases, pubmed-meshheading:8765750-Tissue Distribution, pubmed-meshheading:8765750-Transcription, Genetic
pubmed:year
1996
pubmed:articleTitle
Isolation and characterization of genomic and cDNA clones encoding mouse senescence marker protein-30 (SMP30).
pubmed:affiliation
Department of Molecular Biology, Tokyo Metropolitan Institute of Gerontology, Japan.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't