Linked Life Data

8765733

Source:http://linkedlifedata.com/resource/pubmed/id/8765733

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1996-11-26
pubmed:abstractText
Extraction of potato cell-wall material with solutions of increasing strength of alkali yielded a xyloglucan-rich fraction which was further purified by anion-exchange chromatography and treatment with alpha-amylase and endogalactanase. Methylation analysis indicated that the purified xyloglucan contained a high percentage of unsubstituted glucosyl residues compared to, for instance, apple xyloglucan, and equal amounts of Xyl-(1-->6)-, Gal-(1-->2)-Xyl(1-->6)-, and Ara-(1-->2)-Xyl-(1-->6)-sidechains. This xyloglucan was degraded with endoglucanase (endoV), purified from Trichoderma viride. The resulting digest was fractionated by BioGel P-2 chromatography, followed by preparative high-performance anion-exchange chromatography of the pentamer to nonamer fractions. The purified oligosaccharides were characterized by monosaccharide analysis, mass spectrometry, and degradation with an exoglucanase. Degradation of potato xyloglucan by another endoglucanase (endoIV) of Trichoderma viride yielded a different set of products. EndoIV released predominantly oligosaccharides with two unbranched glucosyl residues at the reducing terminus, whereas endoV also released products containing unbranched glucosyl residues on both ends of the molecule. A difference in the mode of action of endoglucanases with xyloglucan-degrading activity is demonstrated.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0008-6215
pubmed:author
pubmed:issnType
Print
pubmed:day
19
pubmed:volume
288
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
219-32
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Potato xyloglucan is built from XXGG-type subunits.
pubmed:affiliation
Wageningen Agricultural University, Department of Food Science, The Netherlands.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't