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rdf:type |
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lifeskim:mentions |
umls-concept:C0005528,
umls-concept:C0017262,
umls-concept:C0029073,
umls-concept:C0033413,
umls-concept:C0040649,
umls-concept:C0067762,
umls-concept:C0071677,
umls-concept:C0185117,
umls-concept:C0205214,
umls-concept:C0220781,
umls-concept:C1136217,
umls-concept:C2911684
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pubmed:issue |
14
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pubmed:dateCreated |
1996-10-1
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pubmed:databankReference |
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pubmed:abstractText |
We studied capsule-defective (Cap-) serogroup B meningococcal mutants created through Tn916 or omega-fragment mutagenesis. The Cap- phenotypes were the results of insertions in three of four linked genes (synX, synC, and synD) involved in CMP-N-acetylneuraminic acid and polysialic acid capsule biosynthesis, and in ctrA the first of four linked genes involved in capsule membrane transport. Mutations in the CMP-N-acetylneuraminic acid biosynthesis genes synX and synC caused defects in lipooligosaccharide sialylation but not mutations in the putative (alpha2 -> 8)-linked polysialyltransferase (synD) or in ctrA. Reverse transcriptase PCR studies indicated that the four biosynthesis genes (synX to -D) and the capsule transport genes (ctr to -D) were separately transcribed as operons. The operons were separated by a 134-bp intergenic region. Primer extension of synX and ctrA demonstrated that transcription of the operons was divergently initiated from adjacent start sites present in the intergenic region. Both transcriptional start sites were preceded by a perfect -10 Pribnow promoter binding region. The synX to -D, but not the ctrA to -D, transcriptional start site was preceded by a sequence bearing strong homology to the consensus sigma 70 -35 promoter binding sequence. Both promoters showed transcriptional activity when cloned behind a lacZ reporter gene in Escherichia coli. Our results confirm the intrinsic relationship between polysialic acid capsule biosynthesis and lipooligosaccharide sialylation pathways in group B Neisseria meningitidis. Our study also suggests that the intergenic region separating the synX to -D and ctrA to -D operons is an important control point for the regulation of group B capsule expression through coordinated transcriptional regulation of the synX to -D and drA to -D promoters.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-1283998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-1398032,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-1550990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-1644747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-1657783,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-1659649,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-1692081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-1708379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-1761236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-2167422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-2404690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-2456365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-2493648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-2691331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-3059994,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-3108388,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-3123263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-3123395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-3130779,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-4208046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-4872442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-4977282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-6135869,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-6237955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-6546423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-7476183,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-7530316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-7711899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-7790063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-781293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-7814319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-7830552,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-7912406,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-7934821,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-7960107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-7968509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-8022265,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-8045888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-8113198,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-8168940,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-8326854,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-8326855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-8326861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-8331087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-8345519,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-8380612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-8598202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-8631701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8763931-8817495
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
178
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4052-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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