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rdf:type |
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lifeskim:mentions |
umls-concept:C0004611,
umls-concept:C0017262,
umls-concept:C0021467,
umls-concept:C0021469,
umls-concept:C0029073,
umls-concept:C0035495,
umls-concept:C0040648,
umls-concept:C0185117,
umls-concept:C1314939,
umls-concept:C1514562,
umls-concept:C2911684
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pubmed:issue |
4
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pubmed:dateCreated |
1996-10-9
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pubmed:databankReference |
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pubmed:abstractText |
In the purple nonsulfur photosynthetic bacterium Rhodobacter sphaeroides the synthesis of components of the photosystem is regulated in response to oxygen tension and light intensity. We have purified and cloned a trans-acting protein (SPB) that binds to the promoter region of the puf operon, which encodes the apoproteins of light-harvesting complex I and the reaction center. The SPB was composed of a single polypeptide with an apparent molecular mass of 15.0 kDa. The nucleotide sequence of the spb gene was determined. The gene encoded 104 amino acid residues, which correspond to a molecular mass of 11.5 kDa. SPB exhibited 53% homology to HvrA in Rhodobacter capsulatus. The deduced amino acid sequence indicated that SPB contained a region with homology to the leucine-zipper motif of c-JUN, a transcription factor in eukaryotes, and SPB also had a DNA-binding domain on the amino-terminal side of the leucine-zipper motif. The leucine-zipper motif of SPB might contribute to the formation of a dimer. Northern analysis indicated that spb was constitutively and monocistronically transcribed in R. sphaeroides, irrespective of growth conditions. Structural and functional differences between SPB and HvrA are discussed.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0032-0781
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
37
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
515-22
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:8759915-Amino Acid Sequence,
pubmed-meshheading:8759915-Base Sequence,
pubmed-meshheading:8759915-Cloning, Molecular,
pubmed-meshheading:8759915-DNA, Bacterial,
pubmed-meshheading:8759915-Gene Expression,
pubmed-meshheading:8759915-Genes, Bacterial,
pubmed-meshheading:8759915-Leucine Zippers,
pubmed-meshheading:8759915-Light,
pubmed-meshheading:8759915-Molecular Sequence Data,
pubmed-meshheading:8759915-Monomeric GTP-Binding Proteins,
pubmed-meshheading:8759915-NM23 Nucleoside Diphosphate Kinases,
pubmed-meshheading:8759915-Nucleoside-Diphosphate Kinase,
pubmed-meshheading:8759915-Oligonucleotide Probes,
pubmed-meshheading:8759915-Operon,
pubmed-meshheading:8759915-Photosynthesis,
pubmed-meshheading:8759915-Proto-Oncogene Proteins c-jun,
pubmed-meshheading:8759915-Rhodobacter sphaeroides,
pubmed-meshheading:8759915-Sequence Homology, Amino Acid,
pubmed-meshheading:8759915-Transcription Factors
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pubmed:year |
1996
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pubmed:articleTitle |
A transcription factor with a leucine-zipper motif involved in light-dependent inhibition of expression of the puf operon in the photosynthetic bacterium Rhodobacter sphaeroides.
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pubmed:affiliation |
Department of Biological Sciences, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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