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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
14
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pubmed:dateCreated |
1996-9-17
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pubmed:databankReference |
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pubmed:abstractText |
SPSF I and II are two cellular proteins which bind specifically to single-stranded DNA. SPSF I and II binding sites are found in the minimal origin of replication of BPV-1 DNA and near the P2 promoter of the cellular c-myc gene. DNA-binding properties of the two proteins to single-stranded oligonucleotides of different lengths and sequences were quantified by determination of DNA-binding constants. The binding constant of SPSF proteins to the lower strand of the BPV-1 origin was determined to be 1.5 x 10(-10) M-1. Peptide sequences derived from purified SPSF I and II revealed the identity of at least one of the SPSF proteins with the so-called HeLa Pur alpha factor. The HeLa Pur alpha factor was identified previously by virtue of its capacity to bind to purine-rich strands of the PUR element found in initiation zones of DNA replication [Bergemann, A.D., Ma,Z.-W. and Johnson, E.M. (1992) Mol. Cell. Biol. 12, 5673-5682]. Expression of the Pur cDNA confirmed the identity of the Pur alpha protein with the 42 kDa SPSF I protein. Analysis of several Pur alpha cDNA clones revealed the existence of an extended 3'-untranslated region in all Pur mRNAs.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8759014-1326653,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8759014-1353951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8759014-1448097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8759014-1545807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8759014-1656277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8759014-1661672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8759014-1668086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8759014-1846806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8759014-2001590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8759014-2216760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8759014-2670548,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8759014-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8759014-3380685,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8759014-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8759014-6319020,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8759014-7592647,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8759014-7657701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8759014-7736569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8759014-7838710,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8759014-7862639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8759014-7916324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8759014-7979254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8759014-8041609,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8759014-8380226,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8759014-8578590
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
24
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2799-806
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8759014-Animals,
pubmed-meshheading:8759014-Base Sequence,
pubmed-meshheading:8759014-Bovine papillomavirus 1,
pubmed-meshheading:8759014-Cattle,
pubmed-meshheading:8759014-Cell Line,
pubmed-meshheading:8759014-Cyclic AMP Response Element-Binding Protein,
pubmed-meshheading:8759014-DNA, Single-Stranded,
pubmed-meshheading:8759014-DNA-Binding Proteins,
pubmed-meshheading:8759014-HeLa Cells,
pubmed-meshheading:8759014-Humans,
pubmed-meshheading:8759014-Introns,
pubmed-meshheading:8759014-Molecular Sequence Data,
pubmed-meshheading:8759014-Protein Binding,
pubmed-meshheading:8759014-RNA, Messenger,
pubmed-meshheading:8759014-Recombinant Proteins,
pubmed-meshheading:8759014-Replication Origin,
pubmed-meshheading:8759014-Spodoptera,
pubmed-meshheading:8759014-Thymus Gland,
pubmed-meshheading:8759014-Transcription Factors
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pubmed:year |
1996
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pubmed:articleTitle |
Characterization of the single-strand-specific BPV-1 origin binding protein, SPSF I, as the HeLa Pur alpha factor.
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pubmed:affiliation |
Institut für Biochemie, Ludwig-Maximilians-Universität München, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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