Linked Life Data

8757979

Source:http://linkedlifedata.com/resource/pubmed/id/8757979

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1996-9-26
pubmed:abstractText
Neutralization of human immunodeficiency virus type 1 by adding antibody after the virus has attached to the host cell (post-attachment neutralization:PAN) was investigated using three rat monoclonal antibodies (MAbs) directed against the outer domain of the membrane protein, gp 120. Two of the MAbs are specific for the CD4-binding site region and one for the V3 loop. MAb ICR39.13g (CD4-binding site region-specific; IgG2b) effected PAN efficiently at temperatures from 4 to 35 degree C. MAb ICR41.1i (V3 loop-specific; IgG2c) effected PAN only at temperatures of 24 degree C and below. This suggests that its V3 epitope is masked by a change in gp 120 which occurs at temperatures > or = 26 degree C, or that the virion function which is inhibited by ICR41.1i and is responsible for neutralization has already operated at > or = 26 degree C. Resistance to neutralization by ICR41.1i occurred within 20 min of shifting the temperature up to 35 degree C. Finally, MAb ICR39.3b (CD4-binding site region-specific; IgG2b) did not give PAN at any temperature, indicating that neutralization can only occur if this MAb binds virus before it attaches to the cell. Thus, these studies identify at least one novel fusion-independent event, the neutralization target of a V3 MAb, which occurs very early in the initial stages of virus-cell interaction.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-1317
pubmed:author
pubmed:issnType
Print
pubmed:volume
77 ( Pt 7)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1397-402
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Varying temperature-dependence of post-attachment neutralization of human immunodeficiency virus type 1 by monoclonal antibodies to gp 120: identification of a very early fusion-independent event as a neutralization target.
pubmed:affiliation
Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't