Linked Life Data

8757978

Source:http://linkedlifedata.com/resource/pubmed/id/8757978

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1996-9-26
pubmed:abstractText
In approximate 10 percent of natural human immunodeficiency virus 1 (HIV-1) vif gene populations, sequences of shortened vif open reading frames with premature stop codons have been found. Here we report the functional analysis of two patient-derived vif genes. Vif45-2 encodes a C terminally truncated Vif protein of only 173 instead of 192 amino acids and additionally contains several rare amino acid substitutions which are in part shared by vifA65-5. HIV-1 pNL4-3-derived recombinant A45-2 and A65-5 virions were fully infectious in H9 cells and human PBMC, both known to be non-permissive for vif-defective HIV-1. Furthermore, A45-2 virions produced in primary human monocyte-derived macrophages were infectious for MT-4 cells. This study unequivocally demonstrates that the C-terminal region (19 amino acids) of the Vif protein is dispensable for Vif function in the in vitro cell culture systems employed. Additionally, we investigated whether the Vif protein might be phosphorylated in vivo and obtained no evidence for this.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-1317
pubmed:author
pubmed:issnType
Print
pubmed:volume
77 ( Pt 7)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1389-95
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Unimpaired function of a naturally occurring C terminally truncated vif gene product of human immunodeficiency virus type 1.
pubmed:affiliation
Forschungsschwerpunkt Angewandte Tumorvirologie, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
pubmed:publicationType
Journal Article