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rdf:type |
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lifeskim:mentions |
umls-concept:C0080194,
umls-concept:C0205169,
umls-concept:C0392756,
umls-concept:C0449432,
umls-concept:C0520500,
umls-concept:C0887913,
umls-concept:C1179435,
umls-concept:C1513371,
umls-concept:C1521797,
umls-concept:C1524073,
umls-concept:C1548799,
umls-concept:C1705248,
umls-concept:C1707391,
umls-concept:C1707719
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pubmed:dateCreated |
1996-9-26
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pubmed:abstractText |
A poorly aphid-transmissible potato virus Y (PVY-PAT) variant emerged after several cycles of mechanical transmission of an initially aphid-transmissible (AT) isolate. Sequence analysis of the N-terminal region of the helper component-proteinase (HC-Pro) gene revealed a Lys to Glu change at a position previously found to abolish the HC-Pro aphid transmission activity in several potyviruses. Two cycles of aphid transmission allowed the virus population to evolve towards an AT form (PVY-ATnew) where a Glu to Lys change was observed. PVY-PAT produced lower amounts of coat protein and the accumulation of its HC-Pro in infected plants decreased from 7 to 28 days post-inoculation, as compared to PVY-ATnew. RT-PCR and restriction analysis showed that the two virus populations co-existed in the PVY-AT isolate and that the AT form was counter-selected during mechanical transmission. These observations suggest that the Lys to Glu substitution leads to decreased stability of HC-Pro resulting in poor transmissions by aphids, and further strengthen the idea that HC-Pro is involved in the accumulation of potyvirus in infected plants.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0022-1317
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
77 ( Pt 7)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1343-7
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:8757973-Amino Acid Sequence,
pubmed-meshheading:8757973-Animals,
pubmed-meshheading:8757973-Aphids,
pubmed-meshheading:8757973-Base Sequence,
pubmed-meshheading:8757973-Capsid,
pubmed-meshheading:8757973-Capsid Proteins,
pubmed-meshheading:8757973-Cysteine Endopeptidases,
pubmed-meshheading:8757973-DNA, Viral,
pubmed-meshheading:8757973-Insect Vectors,
pubmed-meshheading:8757973-Molecular Sequence Data,
pubmed-meshheading:8757973-Plant Diseases,
pubmed-meshheading:8757973-Potyvirus,
pubmed-meshheading:8757973-Selection, Genetic,
pubmed-meshheading:8757973-Solanum tuberosum,
pubmed-meshheading:8757973-Viral Proteins
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pubmed:year |
1996
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pubmed:articleTitle |
Switches in the mode of transmission select for or against a poorly aphid-transmissible strain of potato virus Y with reduced helper component and virus accumulation.
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pubmed:affiliation |
Laboratoire de biologie Cellulaire, INRA, 78026 Versailles, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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