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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1996-10-9
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pubmed:databankReference |
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pubmed:abstractText |
This study describes a PCR-based approach for the production of a rationally attenuated mutant of Yersinia pestis. Degenerate primers were used to amplify a fragment encoding 91.45% of the aroA gene of Y. pestis MP6 which was cloned into pUC18. The remainder of the gene was isolated by inverse PCR. The gene was sequenced and a restriction map was generated. The Y. pestis aroA gene had 75.9% identity with the aroA gene of Yersinia enterocolitica. The cloned gene was inactivated in vitro and reintroduced into Y. pestis strain GB using the suicide vector pGP704. A stable aro-defective mutant. Y. pestis GB aroA, was isolated and its virulence was examined in vivo. The mutant was attenuated in guinea-pigs and capable of inducing a protective immune response against challenge with the virulent Y. pestis strain GB. Unusually for an aro-defective mutant, the Y. pestis aroA mutant was virulent in mice, with a median dose which induced morbidity of death similar to that of the wild-type, although time to death was significantly prolonged.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1350-0872
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
142 ( Pt 7)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1847-53
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8757748-3-Phosphoshikimate 1-Carboxyvinyltransferase,
pubmed-meshheading:8757748-Alkyl and Aryl Transferases,
pubmed-meshheading:8757748-Amino Acid Sequence,
pubmed-meshheading:8757748-Animals,
pubmed-meshheading:8757748-Bacterial Vaccines,
pubmed-meshheading:8757748-Base Sequence,
pubmed-meshheading:8757748-DNA, Bacterial,
pubmed-meshheading:8757748-DNA Primers,
pubmed-meshheading:8757748-Disease Models, Animal,
pubmed-meshheading:8757748-Female,
pubmed-meshheading:8757748-Guinea Pigs,
pubmed-meshheading:8757748-Mice,
pubmed-meshheading:8757748-Mice, Inbred BALB C,
pubmed-meshheading:8757748-Molecular Sequence Data,
pubmed-meshheading:8757748-Mutagenesis,
pubmed-meshheading:8757748-Mutation,
pubmed-meshheading:8757748-Plague,
pubmed-meshheading:8757748-Sequence Homology, Amino Acid,
pubmed-meshheading:8757748-Transferases,
pubmed-meshheading:8757748-Vaccines, Attenuated,
pubmed-meshheading:8757748-Virulence,
pubmed-meshheading:8757748-Yersinia pestis
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pubmed:year |
1996
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pubmed:articleTitle |
An aroA mutant of Yersinia pestis is attenuated in guinea-pigs, but virulent in mice.
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pubmed:affiliation |
Chemical and Biological Defence Establishment, Salisbury, Wiltshire, UK. 100432.3200@compuserve.com
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pubmed:publicationType |
Journal Article,
Comparative Study
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