Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1996-11-22
|
| pubmed:abstractText |
The IL-4R exists in two forms, either membrane bound or as a soluble (s) molecule. Since the sIL-4R binds to its ligand with high affinity, thereby acting as an immunoregulatory molecule, we were interested in the processes leading to its release. First, the release of sIL-4R in the model of murine leishmaniosis was analyzed. Infection of mice with Leishmania major resulted in up-regulation of sIL-4R production by Ag-stimulated CD4+ T cells, with a maximum around 7 days after infection. To clarify the mechanisms underlying sIL-4R release, in vitro studies were performed. After stimulation of naive lymphoid cells with IL-4, sIL-4R release was dependent on up-regulation of spliced IL-4R mRNA, as shown by inhibition with specific antisense oligonucleotides. In contrast to this, no increase in the spliced IL-4R mRNA and no inhibitory influence of antisense oligonucleotides were observed after stimulation of T cells from IL-4-deficient mice with anti-CD3 mAb. Thus, TCR stimulation can lead to IL-4-independent sIL-4R production. Under these conditions proteolytic shedding of membrane-bound IL-4R appears to be the principal mechanism of release, since in contrast to stimulation with IL-4, iodinated sIL-4R could only be immunoprecipitated after cell surface labeling and subsequent TCR stimulation. The common gamma-chain, a component of the IL-4R complex, did not appear to be involved in the pathways leading to sIL-4R expression. This analysis suggests the existence of two differentially regulated pathways of sIL-4R release, possibly having different consequences for the regulation of IL-4 bioactivity.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-4
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
157
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1846-53
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8757301-Alternative Splicing,
pubmed-meshheading:8757301-Animals,
pubmed-meshheading:8757301-Antigens, CD,
pubmed-meshheading:8757301-Female,
pubmed-meshheading:8757301-Interleukin-4,
pubmed-meshheading:8757301-Leishmaniasis, Cutaneous,
pubmed-meshheading:8757301-Lymphocyte Activation,
pubmed-meshheading:8757301-Mice,
pubmed-meshheading:8757301-Mice, Inbred BALB C,
pubmed-meshheading:8757301-Mice, Inbred C3H,
pubmed-meshheading:8757301-Mice, Inbred C57BL,
pubmed-meshheading:8757301-Mice, Inbred CBA,
pubmed-meshheading:8757301-Mice, Inbred DBA,
pubmed-meshheading:8757301-Mice, SCID,
pubmed-meshheading:8757301-RNA, Messenger,
pubmed-meshheading:8757301-Receptors, Interleukin,
pubmed-meshheading:8757301-Receptors, Interleukin-4,
pubmed-meshheading:8757301-Solubility,
pubmed-meshheading:8757301-T-Lymphocytes
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Two distinct stimulus-dependent pathways lead to production of soluble murine interleukin-4 receptor.
|
| pubmed:affiliation |
Institute of Clinical Microbiology and Immunology, University of Erlangen-Nurnberg, Germany.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|