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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6592
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pubmed:dateCreated |
1996-9-18
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pubmed:abstractText |
The WW domain is a new protein module with two highly conserved tryptophans that binds proline-rich peptide motifs in vitro. It is present in a number of signalling and regulatory proteins, often in several copies. Here we investigate the solution structure of the WW domain of human YAP65 (for Yes kinase-associated protein) in complex with proline-rich peptides containing the core motif PPxY. The structure of the domain with the bound peptide GTPPPPYTVG is a slightly curved, three-stranded, antiparallel beta-sheet. Two prolines pack against the first tryptophan, forming a hydrophobic buckle on the convex side of the sheet. The concave side has three exposed hydrophobic residues (tyrosine, tryptophan and leucine) which form the binding site for the ligand. A non-conserved isoleucine in the amino-terminal flanking region covers a hydrophobic patch and stabilizes the WW domain of human YAP65 in vitro. The structure of the WW domain differs from that of the SH3 domain and reveals a new design for a protein module that uses stacked aromatic surface residues to arrange a binding site for proline-rich peptides.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0028-0836
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
382
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
646-9
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8757138-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:8757138-Amino Acid Sequence,
pubmed-meshheading:8757138-Animals,
pubmed-meshheading:8757138-Binding Sites,
pubmed-meshheading:8757138-Carrier Proteins,
pubmed-meshheading:8757138-Consensus Sequence,
pubmed-meshheading:8757138-Humans,
pubmed-meshheading:8757138-Mice,
pubmed-meshheading:8757138-Models, Molecular,
pubmed-meshheading:8757138-Molecular Sequence Data,
pubmed-meshheading:8757138-Peptides,
pubmed-meshheading:8757138-Phosphoproteins,
pubmed-meshheading:8757138-Proline,
pubmed-meshheading:8757138-Proline-Rich Protein Domains,
pubmed-meshheading:8757138-Protein Binding,
pubmed-meshheading:8757138-Protein Conformation,
pubmed-meshheading:8757138-Protein Structure, Secondary,
pubmed-meshheading:8757138-Sequence Homology, Amino Acid
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pubmed:year |
1996
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pubmed:articleTitle |
Structure of the WW domain of a kinase-associated protein complexed with a proline-rich peptide.
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pubmed:affiliation |
European Molecular Biology Laboratory, Heidelberg, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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