Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
|
| pubmed:dateCreated |
1996-9-17
|
| pubmed:abstractText |
We studied the localization of latent transforming growth factor-beta 1 (TGF-beta 1) and its binding protein (LTBP-1) in the extracellular matrix of cultured human fibroblasts by immunofluorescence and immunoelectron microscopy. Immunofluorescence of confluent fibroblast cultures indicated that LTBP-1 localizes to extracellular fibrillar structures resembling fibronectin-collagen matrix. Similar fibrillar structures were detected in cells stained with antibodies specific for TGF-beta 1 propeptide (beta 1-LAP). Both LTBP-1 and beta 1-LAP colocalized with fibronectin in double immunofluorescence analysis. These fibrillar structures were resistant to extraction with sodium deoxycholate, which is further evidence that LTBP-1 and large latent TGF-beta 1 complexes are integral components of the extracellular matrix. SV-40-transformed human fibroblasts lacked extracellular LTBP-1 fibers. EM analysis revealed approximately 10-nm-thick microfibrils that were labeled by anti-LTBP at 90-140-nm intervals. In addition, LTBP-1 was found in structures that were heavily labeled for fibronectin. The accumulation of LTBP-1 in the fibronectin matrix could be reconstituted in vitro. When isolated matrix components were immobilized on nitrocellulose and incubated with fibroblast conditioned medium, LTBP-1 from the medium associated with cellular fibronectin but not with heparan or chondroitin sulfate, vitronectin, tenascin, laminin, or collagen I or IV. The association of LTBP-1 with cellular fibronectin was abolished by treatment of the medium with plasmin, which cleaves LTBP-1 and inhibits its assembly to matrix. The present results indicate that latent TGF-beta 1 complexes are components of the extracellular matrix and suggest that alterations of the pericellular matrix could result in aberrant TGF-beta signaling.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Collagen,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Conditioned,
http://linkedlifedata.com/resource/pubmed/chemical/Fibronectins,
http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/LTBP1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Latent TGF-beta Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta1
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0022-1554
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
44
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
875-89
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8756760-Carrier Proteins,
pubmed-meshheading:8756760-Cell Transformation, Viral,
pubmed-meshheading:8756760-Cells, Cultured,
pubmed-meshheading:8756760-Collagen,
pubmed-meshheading:8756760-Culture Media, Conditioned,
pubmed-meshheading:8756760-Extracellular Matrix,
pubmed-meshheading:8756760-Fibroblasts,
pubmed-meshheading:8756760-Fibronectins,
pubmed-meshheading:8756760-Fluorescent Antibody Technique,
pubmed-meshheading:8756760-Humans,
pubmed-meshheading:8756760-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:8756760-Latent TGF-beta Binding Proteins,
pubmed-meshheading:8756760-Microscopy, Immunoelectron,
pubmed-meshheading:8756760-Peptide Fragments,
pubmed-meshheading:8756760-Protein Binding,
pubmed-meshheading:8756760-Protein Precursors,
pubmed-meshheading:8756760-Proteins,
pubmed-meshheading:8756760-Simian virus 40,
pubmed-meshheading:8756760-Transforming Growth Factor beta,
pubmed-meshheading:8756760-Transforming Growth Factor beta1
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Latent transforming growth factor-beta 1 and its binding protein are components of extracellular matrix microfibrils.
|
| pubmed:affiliation |
Departments of Virology, University of Helsinki, Finland.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|