Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1996-11-22
|
| pubmed:abstractText |
Transformed murine fetal thymocyte cell lines were derived by incubating fetal thymic organ cultures with a v-myc/v-raf-containing retroviral construct in order to model developmental stages within the early triple negative (CD3-CD4-CD8-) thymocyte population. The resulting 10 cell lines had a lymphoid morphology, were all CD44+, CD90+, and were triple negative by surface antigen analysis. The cell lines, however, were distinguishable by differences in the expression of T cell-associated and T cell-specific genes. The CD3 genes were observed to be discoordinately expressed, in that CD3 gamma chain gene expression was detected in 2 cell lines in the absence of CD3 delta and epsilon expression. Expression of the CD3 gamma chain gene was observed in cell lines without the expression of other T cell-specific genes or T cell receptor rearrangement and may be one of the earliest T cell-specific genes to be expressed. The transcription factor Ikaros was expressed in all 10 cell lines, whereas the transcription factor TCF1 alpha was expressed only in the 2 most differentiated lines. In 8 cell lines, expression of partial TCR beta and/or TCR alpha transcripts was observed by Northern blot. In several lines, expression of rearranged TCR alpha transcripts in the absence of TCR beta transcripts was demonstrated; however, TCR beta DJ rearrangements were observed by Southern blot in all but 1 of these cell lines. Thus, these cell lines, ordered based on the general pattern of additive gene expression observed, may reflect various stages of triple-negative thymocyte differentiation and provide an in vitro mechanism to elucidate some of the molecular events involved in early thymocyte development.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0008-8749
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
10
|
| pubmed:volume |
171
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
41-7
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:8754860-Animals,
pubmed-meshheading:8754860-Antigens, CD,
pubmed-meshheading:8754860-Blotting, Northern,
pubmed-meshheading:8754860-Blotting, Southern,
pubmed-meshheading:8754860-Cell Differentiation,
pubmed-meshheading:8754860-Cell Line, Transformed,
pubmed-meshheading:8754860-Cell Transformation, Viral,
pubmed-meshheading:8754860-Fetus,
pubmed-meshheading:8754860-Gene Rearrangement, T-Lymphocyte,
pubmed-meshheading:8754860-Genes, myc,
pubmed-meshheading:8754860-Immunophenotyping,
pubmed-meshheading:8754860-Mice,
pubmed-meshheading:8754860-Organ Culture Techniques,
pubmed-meshheading:8754860-Polymerase Chain Reaction,
pubmed-meshheading:8754860-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:8754860-Receptors, Antigen, T-Cell, gamma-delta,
pubmed-meshheading:8754860-T-Lymphocytes,
pubmed-meshheading:8754860-Thymus Gland,
pubmed-meshheading:8754860-Virus Integration
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Development and characterization of v-myc/v-raf-transformed murine fetal thymocyte cell lines.
|
| pubmed:affiliation |
Department of Cellular Pathology, Armed Forces Institute of Pathology, Washington, DC 20306, USA.
|
| pubmed:publicationType |
Journal Article
|