Linked Life Data

8754681

Source:http://linkedlifedata.com/resource/pubmed/id/8754681

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1996-10-21
pubmed:abstractText
C6-aldehydes are synthesized via lipoxygenase/hydroperoxide lyase action on polyunsaturated fatty acid (PUFA) substrates in plant leaves. The source pools and subcellular location of the processes are unknown. A close relationship is found between the composition of PUFA and the composition of C6-aldehydes. In the current study, this relationship was tested using the Arabidopsis PUFA mutant lines act1, fad2, fad3, fad5, fad6, and fad7. The results indicate that C6-aldehyde formation is influenced by the alteration of C18 PUFA levels. Mutants act1 and fad5, which are deficient in C16 unsaturated fatty acids, had wild-type levels of C6-aldehyde production. Mutants deficient in the chloroplast hexadecenoic acid/oleic acid desaturase (fad6) or hexadecadienoic acid/linoleic acid desaturase (fad7) had altered C6-aldehyde formation in a pattern similar to the changes in the PUFA. Mutations that impair phosphatidylcholine desaturase activity, such as fad2 and fad3, however, resulted in increased E-2-hexenal formation. The enzymes involved in C6-aldehyde production were partially characterized, including measurement of pH optima. The differences in C6-aldehyde formation among the fatty acid mutants of Arabidopsis appeared not to result from alteration of lipoxygenase/hydroperoxide lyase pathway enzymes. Investigation of the fatty acid composition in leaf phospholipids, glycolipids, and neutral lipids and analysis of the fatty acid composition of chloroplast and extrachloroplast lipids indicate that chloroplasts and glycolipids of chloroplasts may be the source or major source of C6-aldehyde formation in Arabidopsis leaves.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8754681-14231719, http://linkedlifedata.com/resource/pubmed/commentcorrection/8754681-16593939, http://linkedlifedata.com/resource/pubmed/commentcorrection/8754681-16653005, http://linkedlifedata.com/resource/pubmed/commentcorrection/8754681-16658772, http://linkedlifedata.com/resource/pubmed/commentcorrection/8754681-16664915, http://linkedlifedata.com/resource/pubmed/commentcorrection/8754681-16666802, http://linkedlifedata.com/resource/pubmed/commentcorrection/8754681-16668490, http://linkedlifedata.com/resource/pubmed/commentcorrection/8754681-1730697, http://linkedlifedata.com/resource/pubmed/commentcorrection/8754681-454635, http://linkedlifedata.com/resource/pubmed/commentcorrection/8754681-7506426, http://linkedlifedata.com/resource/pubmed/commentcorrection/8754681-8290626
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0032-0889
pubmed:author
pubmed:issnType
Print
pubmed:volume
111
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
805-12
pubmed:dateRevised
2010-9-13
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
The impact of alteration of polyunsaturated fatty acid levels on C6-aldehyde formation of Arabidopsis thaliana leaves.
pubmed:affiliation
Department of Agronomy, University of Kentucky, Lexington 40546, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't