Linked Life Data

8749803

Source:http://linkedlifedata.com/resource/pubmed/id/8749803

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1996-9-26
pubmed:abstractText
We studied the effect a variety of hormones and chemical stimuli on the activity of low Km aldehyde dehydrogenase (ALDH) in rat H4IIEC3 hepatoma cells and ALDH activity in human HuH7 hepatoma cells. The low Km enzyme in H4IIEC3 cells reflects ALDH2 activity, and the ALDH activity in HuH7 likely represents ALDH5. Of the steroid hormone family, thyroid hormone, progesterone, and dihydrotestosterone increased low Km ALDH activity approximately 50%, whereas dexamethasone and estradiol had little effect. Insulin decreased the activity of low Km ALDH. None of these hormones affected the activity of ALDH in HuH7 cells. Among second messengers, 8-bromo-cAMP and A23187 increased low Km ALDH activity; HuH7 ALDH activity again was unchanged. Exposure of the cells to 22 mM ethanol reduced low Km activity by approximately 20%, whereas hydrogen peroxide, tumor necrosis factor-alpha, and interleukin-1 beta had little effect. Ultraviolet light increased the HuH7 ALDH activity. Retinaldehyde or retinolc acid reduced the HuH7 ALDH activity, but had no effect on low Km ALDH activity. These data suggest that low Km ALDH2 can be regulated by hormones and may not be constitutive as previously thought, and that the HuH7 ALDH is regulated differently.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0145-6008
pubmed:author
pubmed:issnType
Print
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1414-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Hormonal and chemical influences on the expression of class 2 aldehyde dehydrogenases in rat H4IIEC3 and human HuH7 hepatoma cells.
pubmed:affiliation
Department of Medicine, Indiana University School of Medicine, Indianapolis 46202-5121, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't