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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1996-11-7
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pubmed:abstractText |
A radioassay has been developed to quantify the uptake and initial metabolism of adenosine (Ado) or deoxyadenosine (dAdo) by human erythrocytes. Cell suspension and [3H]Ado are mixed at 3-s intervals with a novel dual-syringe apparatus, and uptake and metabolism of Ado is stopped by centrifuging the cells through a dibutylphthalate layer into perchloric acid. The neutralized cell extract is analyzed by two-dimensional chromatography on poly(ethyleneimine)-cellulose plates by two procedures using combinations of solvents optimised for the separation of nucleosides and nucleobases, and for nucleotides derived from the exogenous [3H]Ado.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1572-6495
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
674
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
132-7
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pubmed:dateRevised |
2007-10-16
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pubmed:meshHeading |
pubmed-meshheading:8749261-Adenosine,
pubmed-meshheading:8749261-Blood Chemical Analysis,
pubmed-meshheading:8749261-Cellulose,
pubmed-meshheading:8749261-Chromatography, Thin Layer,
pubmed-meshheading:8749261-Deoxyadenosines,
pubmed-meshheading:8749261-Dibutyl Phthalate,
pubmed-meshheading:8749261-Erythrocytes,
pubmed-meshheading:8749261-Humans,
pubmed-meshheading:8749261-Kinetics,
pubmed-meshheading:8749261-Perchloric Acid,
pubmed-meshheading:8749261-Polyethyleneimine
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pubmed:year |
1995
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pubmed:articleTitle |
Rapid radioassay for metabolites of adenosine and deoxyadenosine in erythrocytes.
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pubmed:affiliation |
Department of Biochemistry, University of Sydney, NSW, Australia.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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