Linked Life Data

8747785

Source:http://linkedlifedata.com/resource/pubmed/id/8747785

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1996-10-8
pubmed:abstractText
Recent metabolic studies suggest that the incretin effect of gut hormones may account for most of the circulating insulin during mild postprandial hyperglycemia after transplantation of isolated islets. As yet, however, insulinotropic effects of pharmacological rather than physiological levels of the incretin candidates cholecystokinin-33 (CCK-33), gastric inhibitory polypeptide (GIP), and glucagon-like peptide-1 (GLP-1) on isolated perifused islets have been reported. We examined the insulinotropic effects of these peptides during perifusion of canine isolated islets. Exploration of beta-cell sensitivity in our model with graded 0.1-10 nM doses of CCK-33 and GIP at a 7.5 mM glucose level demonstrated insulinotropic effects from the lowest level. We, therefore, focused on the (near-) physiological effects of both CCK-33 (20 pM), GIP (500 pM), and GLP-1 (100 pM) during perifusion at a 2.5, 7.5, and 10 mM glucose level. No effects of CCK-33 were observed. GIP enhanced insulin release 1.1- and 1.2-fold, at the 7.5 and 10 mM glucose level, respectively. GLP-1 stimulated insulin output from the 2.5 mM glucose level; and a maximum, 2-fold, increase of insulin output was observed from the 7.5 mM glucose level. Thus, isolated perifused islets do respond to near-physiological beta-cell stimulation with gut hormones, and both GIP and GLP-1 may contribute to a hyperglycemia-enhanced activation of the enteroinsular axis after transplantation of isolated islets.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0167-0115
pubmed:author
pubmed:issnType
Print
pubmed:day
7
pubmed:volume
60
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
61-7
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Insulinotropic effects of cholecystokinin, gastric inhibitory polypeptide and glucagon-like peptide-1 during perifusion of short-term cultured canine isolated islets.
pubmed:affiliation
Department of Surgery, University Hospital, Leiden, Netherlands. MvdBurg@RULLF2.MedFac.LeidenUniv.NL
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't