Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1996-10-24
pubmed:abstractText
Genetic factors may be of importance in determining inter-individual susceptibility to alcoholic liver disease (ALD). Among the candidate genes which have been considered to be important are those which code for enzymes involved in alcohol metabolism. Cytochrome P4502E1 (CYP2E1) metabolizes alcohol to acetaldehyde and the hydroxyethyl radical, and is also inducible by alcohol. A Rsa I restriction fragment length polymorphism (RFLP) in the 5'-flanking region of the CYP2E1 gene has been identified by other investigators, studies showing that the mutant allele (termed c2) shows greater transcriptional activity, higher protein levels and increased activity compared with the wild-type allele (c1). We have used PCR-RFLP analysis to determine whether the frequency of these alleles differed in 95 Caucasian patients with ALD compared with 205 control subjects (comprising 58 alcoholics with no liver disease, 47 patients with non-alcoholic liver disease and 100 healthy volunteers). In controls, the frequency (0.024) of the c2 allele was similar to that previously reported in other Caucasian populations. The c2 allele frequency in patients with ALD (0.1), however, was significantly (p = 0.0003; odds ratio (OR) 4.5, 95% CI 1.9-10.9) higher than in control subjects. The findings indicate that Caucasians carrying the Rsa I c2 allele of the CYP2E1 gene may be at higher risk of developing ALD if they abuse alcohol.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0960-314X
pubmed:author
pubmed:issnType
Print
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
351-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:8747406-Alcoholism, pubmed-meshheading:8747406-Alleles, pubmed-meshheading:8747406-Base Sequence, pubmed-meshheading:8747406-Case-Control Studies, pubmed-meshheading:8747406-Cytochrome P-450 CYP2E1, pubmed-meshheading:8747406-Cytochrome P-450 Enzyme System, pubmed-meshheading:8747406-DNA Primers, pubmed-meshheading:8747406-Ethanol, pubmed-meshheading:8747406-European Continental Ancestry Group, pubmed-meshheading:8747406-Female, pubmed-meshheading:8747406-Gene Frequency, pubmed-meshheading:8747406-Humans, pubmed-meshheading:8747406-Liver Diseases, Alcoholic, pubmed-meshheading:8747406-Male, pubmed-meshheading:8747406-Molecular Sequence Data, pubmed-meshheading:8747406-Odds Ratio, pubmed-meshheading:8747406-Oxidoreductases, N-Demethylating, pubmed-meshheading:8747406-Pharmacogenetics, pubmed-meshheading:8747406-Polymerase Chain Reaction, pubmed-meshheading:8747406-Polymorphism, Restriction Fragment Length, pubmed-meshheading:8747406-Risk Factors
pubmed:year
1995
pubmed:articleTitle
Genetic polymorphism of cytochrome P4502E1 and risk of alcoholic liver disease in Caucasians.
pubmed:affiliation
Department of Pharmacology and Therapeutics, University of Liverpool, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't