Linked Life Data

8746913

Source:http://linkedlifedata.com/resource/pubmed/id/8746913

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1996-9-26
pubmed:abstractText
A new method is described for the controlled and specific depletion of calcium from the vascularly perfused heart of experimental animals by means of dialysis, using a pericardial solution. A 30-40 ml isotonic phosphate buffer pH 7.3 with a low Ca2+ and high Mg2+ concentration (0.2 and 2.7 mM respectively) was inserted into the pericardial cavity of anaesthetized dogs and kept there for 10 or 60 min. The calcium content of the subendocardial and subepicardial halves of the left ventricular wall was similarly decreased to about 70% (P < 0.01) within 10 min and to 62% (P < 0.001) at 60 min, compared to that of hearts dialysed for 60 min in a standard solution of Ca2+ 1.2 mM and Mg2+ 1 mM. Calcium content of the myocardium dialysed with low Ca2+ and a standard Mg2+ solution decreased to only 75% (P < 0.01) at 60 min. Similar changes of calcium were measured in other parts of the heart. An increase in Ca2+ concentration in the pericardial solution was observed at the same time as a decrease in calcium in the myocardium. The increase in Ca2+ reached about 0.7 mM at 60 min, but decreased slightly, and finally, fell to 85% of pre-dialysis values at 60 min. It is concluded that this method of myocardial dialysis is effective in reducing myocardial calcium and is influenced by the duration of dialysis and the Mg2+ content of dialysate.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0195-668X
pubmed:author
pubmed:issnType
Print
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1430-4
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Rapid diminution of calcium in the myocardium by transfer to pericardial solutions. Myocardial dialysis.
pubmed:affiliation
University of Athens, School of Medicine, Department of Clinical Therapeutics, Greece.
pubmed:publicationType
Journal Article