Linked Life Data

8745932

Source:http://linkedlifedata.com/resource/pubmed/id/8745932

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1996-10-30
pubmed:abstractText
As conventional methods for the detection and/or diagnosis of infections with Leishmania parasites have limitations, the DNA-based alternatives have received much attention. By targeting multi-copy sequences such as kinetoplast DNA, ribosomal RNA genes, mini-exon-derived RNA genes or genomic repeats, the sensitivity of these systems can be increased. Similarly, by targeting conserved or variable regions of these targets, the specificity can be tailored to the genus, complex, species or even the individual isolate level. There are two main approaches to DNA-based detection: DNA probes involving hybridization; and amplification approaches such as PCR. DNA probes are less sensitive than amplification but are useful for large-scale screening of sandfly vectors or reservoir hosts, for example. PCR is much more sensitive and has been used for patient diagnosis with a sensitivity greater than microscopy or culture. The application of DNA probes and PCR can be simplified using chemiluminescent or colorimetric end-points, respectively, but both techniques require some specialized equipment and a certain degree of technical expertise. For this reason, their use is limited to research laboratories or central diagnostic facilities.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0003-4983
pubmed:author
pubmed:issnType
Print
pubmed:volume
89 Suppl 1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
95-100
pubmed:dateRevised
2009-5-18
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
DNA-based methods in the detection of Leishmania parasites: field applications and practicalities.
pubmed:affiliation
London School of Hygiene and Tropical Medicine, U.K.
pubmed:publicationType
Journal Article, Review, Research Support, Non-U.S. Gov't