Linked Life Data

8745247

Source:http://linkedlifedata.com/resource/pubmed/id/8745247

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1996-10-24
pubmed:abstractText
An assay is described for the quantitative detection of excretory/secretory antigens liberated by the intestinal nematode Heligmosomoides polygyrus and voided in the host's faeces. A rabbit polyclonal antiserum to the antigens detected them in a dose-dependent manner and was sufficiently sensitive to recognise infection in mice carrying a mean worm burden of nine worms. The assay was specific, giving higher optical density readings with the faeces of mice infected with H polygyrus than with faeces from mice carrying patent infections with Trichuris muris, Hymenolepsis microstoma or the intestinal phase of Trichinella spiralis, or with faeces from rats carrying Hymenolepis diminuta or hamsters carrying Necator americanus or Ancylostoma ceylanicum. It detected the parasite antigens in faeces stored for eight weeks at -20, 4 or 20 degrees C and could detect prepatent infections. The assay has potential for the development of infection intensity-sensitive assays for gastrointestinal nematodes and for the detection of animals harbouring the arrested larvae of parasites.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0034-5288
pubmed:author
pubmed:issnType
Print
pubmed:volume
60
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7-12
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Detection of gastrointestinal nematodes by a coproantigen capture ELISA.
pubmed:affiliation
Department of Life Science, University of Nottingham.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't