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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1977-8-12
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pubmed:abstractText |
Cytochrome P-450 content and p-nitroanisole demethylation (a mixed-function oxidation) were investigated in the microsomal fraction from rabbit alveolar macrophages. The content of cytochrome P-450 was 0.13 +/- 0.024 (mean +/- S.E., n = 9) nmol/mg protein and was not stimulated by pretreatment of rabbits with chlorpromazine. Pretreatment with BCG resulted in decreased cytochrome P-450-specific content, suggesting that the microsomal protein pool was diluted by de novo synthesis of noncytochrome proteins. Demethylation of p-nitroanisole by alveolar macrophage microsomes during a 1 hr incubation was 17.1 +/- 1.4 nmol X hr-1 X mg protein-1. The microsomal fraction from homogenates of whole lungs had a cytochrome P-450 content of 0.32 +/- 0.078 nmol/mg protein and p-nitroanisole demethylase activity of 26.6 +/- 8.7 nmol X hr-1 X mg protein-1. The results indicate the presence of cytochrome P-450 in rabbit alveolar macrophages and show that the microsomal fraction can catalyze a mixed-function oxidation reaction. Comparison of alveolar macrophage and whole lung microsomal preparations indicates that alveolar macrophage cytochrome P-450 comprises a minor fraction of the total pulmonary pool.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome P-450 Enzyme System,
http://linkedlifedata.com/resource/pubmed/chemical/Mixed Function Oxygenases,
http://linkedlifedata.com/resource/pubmed/chemical/Nitroanisole O-Demethylase,
http://linkedlifedata.com/resource/pubmed/chemical/Oxidoreductases
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0022-2143
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
90
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
101-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:874360-Animals,
pubmed-meshheading:874360-Cytochrome P-450 Enzyme System,
pubmed-meshheading:874360-Light,
pubmed-meshheading:874360-Lung,
pubmed-meshheading:874360-Macrophages,
pubmed-meshheading:874360-Male,
pubmed-meshheading:874360-Microsomes,
pubmed-meshheading:874360-Mixed Function Oxygenases,
pubmed-meshheading:874360-Nitroanisole O-Demethylase,
pubmed-meshheading:874360-Oxidoreductases,
pubmed-meshheading:874360-Pulmonary Alveoli,
pubmed-meshheading:874360-Rabbits,
pubmed-meshheading:874360-Spectrophotometry
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pubmed:year |
1977
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pubmed:articleTitle |
Cytochrome P-450 content and mixed-function oxidation by microsomes from rabbit alveolar macrophages.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.
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