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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1996-10-4
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pubmed:abstractText |
The early conceptus (embryo and associated membranes) of domestic ruminats signals its presence to the maternal uterus through production of interferon-tau (IFN-tau). Production of IFN-tau ensures continued production of progesterone, the hormone of pregnancy, by the ovarian corpus luteum. This paper reports the high-level expression and efficient secretion of biologically active recombinant ovine IFN-tau (rOvIFN-tau) by Pichia pastoris. The developed method produces more than 80% pure recombinant ovine IFN-tau, obviating the need for further purification for many purposes. Initial fermentation studies produced IFN-tau at 280 mg/liter and demonstrate the potential of this system for large-scale production of IFN-tau.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1079-9907
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
119-26
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pubmed:dateRevised |
2006-5-1
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pubmed:meshHeading |
pubmed-meshheading:8742364-Animals,
pubmed-meshheading:8742364-Base Sequence,
pubmed-meshheading:8742364-Cell Line, Transformed,
pubmed-meshheading:8742364-Chromosome Mapping,
pubmed-meshheading:8742364-Culture Media,
pubmed-meshheading:8742364-Fermentation,
pubmed-meshheading:8742364-Interferon Type I,
pubmed-meshheading:8742364-Molecular Sequence Data,
pubmed-meshheading:8742364-Pichia,
pubmed-meshheading:8742364-Plasmids,
pubmed-meshheading:8742364-Pregnancy Proteins,
pubmed-meshheading:8742364-Recombinant Proteins,
pubmed-meshheading:8742364-Secretory Rate,
pubmed-meshheading:8742364-Sheep
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pubmed:year |
1996
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pubmed:articleTitle |
High yield expression and secretion of the ovine pregnancy recognition hormone interferon-tau by Pichia pastoris.
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pubmed:affiliation |
Department of Animal Science, Texas A&M University, College Station 77843-2471, USA.
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pubmed:publicationType |
Journal Article
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