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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1996-10-11
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pubmed:abstractText |
Recombinant chicken stem cell factor (SCF) was produced in bacteria as a histidine-tagged protein (His delta SCF) and purified by affinity chromatography on an Ni(2+)-NTA agarose column. His delta SCF is devoid of signal peptide and transmembrane domains and therefore mimics a soluble form of SCF. We demonstrate that the factor is biologically fully active in supporting sustained proliferation of SCF-dependent red blood progenitor cells of chicken in vitro. Polyclonal SCF-specific antibodies were raised in rabbits and shown to display potent SCF-neutralizing activity. Thus, recombinant chicken His delta SCF together with the SCF-specific antibody provide valuable tools for studying the role of SCF and c-kit receptor in development, growth and differentiation of avian haematopoietic cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1043-4666
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
8
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
14-20
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:8742062-Amino Acid Sequence,
pubmed-meshheading:8742062-Animals,
pubmed-meshheading:8742062-Antibodies,
pubmed-meshheading:8742062-Cell Differentiation,
pubmed-meshheading:8742062-Cell Division,
pubmed-meshheading:8742062-Cell Line,
pubmed-meshheading:8742062-Chickens,
pubmed-meshheading:8742062-DNA Primers,
pubmed-meshheading:8742062-Erythrocytes,
pubmed-meshheading:8742062-Hematopoietic Stem Cells,
pubmed-meshheading:8742062-Histidine,
pubmed-meshheading:8742062-Molecular Sequence Data,
pubmed-meshheading:8742062-Neutralization Tests,
pubmed-meshheading:8742062-Polymerase Chain Reaction,
pubmed-meshheading:8742062-Proto-Oncogene Proteins c-kit,
pubmed-meshheading:8742062-Rabbits,
pubmed-meshheading:8742062-Recombinant Proteins,
pubmed-meshheading:8742062-Sequence Tagged Sites,
pubmed-meshheading:8742062-Stem Cell Factor,
pubmed-meshheading:8742062-Thymidine,
pubmed-meshheading:8742062-Transfection
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pubmed:year |
1996
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pubmed:articleTitle |
Avian stem cell factor (SCF): production and characterization of the recombinant His-tagged SCF of chicken and its neutralizing antibody.
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pubmed:affiliation |
Institute of Molecular Pathology, Vienna, Austria.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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